FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_44_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_44_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204424
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC100.007086801144.99976145
GTATATG1701.0256693E-425.5944541
GCATAGA1450.001221374525.0060751
TACATTG1450.001223118624.9999585
GGGGTAG1550.001801044123.392781
ATACCAT1952.6049203E-422.3076536
GGCCACA2104.2980246E-420.714257
TGAACAG2205.8814127E-419.7726965
TAATACT1850.005033463219.594564
GGACAGT1850.005033463219.594566
GGACTGC1900.005872276619.0789156
ACAGTGC1950.00682246518.589718
ACACTGT2359.1660826E-418.5106076
TAGACAG3201.9157858E-518.124975
CATACTG2000.00789519118.124975
GCCACAC2050.009102385517.6828988
TACACAG2800.00296064215.5356885
TATATAC3304.9706E-415.3787623
TTGAACA2900.003739917714.9999759
TACCATT3000.004685136514.4999757
Produced by FastQC (version 0.11.5)