FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_35_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_35_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143838
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTATC657.3155656E-444.6153878
CCTACTC800.002030831836.253
GGGGCTA800.002030831836.251
TACCTAT900.003617988432.222227
GAGTTAT1153.1487533E-431.521741
GTACCAG950.004713989330.5263181
ACCTATG1204.04096E-430.2083347
GTATATG1358.048927E-426.8518521
ACATGTA2000.00788402618.1255
AGACGTC500.001392878517.40000230-34
AATCCCA3850.001591545813.1818196
TTTATAT4700.00708078910.7978733
TTCCATT11602.9828789E-58.1250017
ATTCCAT9950.00376196867.28643236