FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_23_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_23_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122643
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAA604.928008E-448.3333321
GGGGCAC855.314011E-542.6470571
GGTATGA850.002732656234.1176451
GGGTAGG1102.4237645E-432.9545481
GAAATAC1453.4657343E-530.03
GTACATA1050.00768253227.6190471
GTATTAG1100.00963915826.3636381
GGGGCAT1450.001220313525.01
GGGGCGC1450.001220313525.01
CCATTCG2950.00417738414.7457639
TATTATT3050.00521258114.2622962