FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137116
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1860.13565156509816506No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACC400.006039798354.3754
GTGTAAG657.3143694E-444.6153831
AGAGCAT1209.503792E-636.256
ATATGTG1851.626122E-731.3513535
CATATAC1000.006056437729.01
TACTGTG1000.006056437729.07
GAGCATC1000.006056437729.07
GTACAGT1050.007685651527.619056
ATATACA1958.682737E-626.0256422
CTCTAAA1500.001487719424.1666681
CTATAAA1801.5109719E-424.1666661
TATATGT2151.850556E-523.604654
ACATATA1650.002589536821.9696968
TAGACAG2400.001053378918.1255
ATGTAAT2550.001581547917.0588231
TATGTGT2550.001581547917.0588236
TACTAAA2950.004180740614.7457642
CCTAGAG1604.4102682E-49.062555-59
CTGCCCC2400.00315012786.6458335100-104
Produced by FastQC (version 0.11.5)