FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151483
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGT657.3168863E-444.6152848
CTAGTGT700.001053812441.428484
TGGTATC800.002031195736.2499167
GCAGTCC850.002734710734.117578
CTGGTCA1402.7314845E-531.071369
GGGCACA1504.3646305E-529.0095121
TAAGGCT1358.051026E-426.851794
AGCAGTC1358.051026E-426.851797
CCTATGG1100.00964629926.3635799
TATACTG1500.001488457724.1666135
GCCCAAG1550.001798008723.3947661
TAATAGT1550.001801468823.3870414
CCCAAGG2155.02903E-420.2325132
GGCCAAG2000.00787100918.1309451
TGCAGGA2000.00788601918.1249582
CCTGTAA3406.2063104E-414.9313651
CGGGTTC600.00470071214.49996840-44
TATATAT9051.526167E-48.8121352
GTGTGTG8700.00100510788.3333147
Produced by FastQC (version 0.11.5)