FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_21_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_21_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2920.10442855763419248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGTA259.379763E-486.999682
AGTCGGC350.003563870262.1426288
GCGGTAA450.00961166948.35911
GCTAGGG1600.002171306622.6561683
GTATATA3854.2200554E-1022.6094471
TCTACAC1650.002596275221.9696163
GTGTACA2704.4055523E-621.4929311
TCCCTAC2053.6574205E-421.2194333
TATAGAG2053.6574205E-421.2194332
GGGTGTA1750.00364097120.7253281
GTATAGA1750.00364097120.7253281
GTCTATA1800.004286196520.1496241
GTCCTAA1900.005859437419.0891191
ATACTGT3252.1938404E-517.8460876
GGCCGGG2050.00908251317.6923521
TTAGACA2050.00911063817.6828614
TCTAGGC2050.00911063817.6828613
TAGAGCA3302.5014968E-517.5756934
TATACTG2901.8593665E-417.4999355
TCAGGGC2550.001587161117.0587623
Produced by FastQC (version 0.11.5)