FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177366
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGT554.0210034E-665.982991
GGCTAGG1051.8354543E-434.562521
ATAGGTG1153.151842E-431.5214945
GTTGTAG1204.018245E-430.2422031
CAGTACA1453.474581E-529.9997674
GGCCCTA1000.006028063629.0325131
GCGTCGA200.006074323328.99977585-89
GTGCACT1050.00769193527.6188344
CTAAGGA1050.00769193527.6188343
GGGGGTA1050.00769193527.6188342
CTATGGC1100.00965092626.363439
TACACAG2151.854651E-523.6044675
GTCTTGC1550.001790933723.4133191
GCTGTAG1600.00215406722.6816521
GTATATA2404.2996013E-521.1695421
CTGAGGA2205.8774423E-419.7725734
CTGAGTG1850.005031060419.5944429
GGGTGAG1950.006774937318.6105861
GTGTAAA1950.006774937318.6105861
ACTGTGT2359.1599324E-418.5104948
Produced by FastQC (version 0.11.5)