FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_17_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_17_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134992
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAACG400.006039593454.3750044
GTATACC553.2090177E-452.7272724
GGGGTAT1053.7995142E-641.428571
GGGTAGA950.00471297230.5263182
TAACCCT950.00471297230.5263186
ACAGTGC1000.00605610629.08
TATACAA1504.370921E-529.05
GGCTTAT1050.00768523327.6190471
TCTACAC1050.00768523327.6190473
GGGGTGC1358.046343E-426.8518521
CTATACA1100.0096425426.3636364
TATTGTC1100.0096425426.3636367
GGGGTAG1100.0096425426.3636361
AGATTGC1100.0096425426.3636366
GGGTTAT1100.0096425426.3636361
GGGGCCT1500.00148759924.1666661
GAGACTG1650.002589327521.9696965
TAATACA1900.005862098719.0789474
GCCACTG1900.005862098719.0789477
GTATTAT1950.00681066818.5897451
Produced by FastQC (version 0.11.5)