FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_178_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222483
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2340.10517657528889847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAT1157.0850547E-637.8514331
TACTAGG1000.006064582628.9998652
TCCATAC1606.804902E-527.1873743
GTATTGG1450.00121867625.0167521
CCTTATA1500.001484823624.1828611
GGACTGT1801.5151556E-424.1665536
CCATACT1550.001804140423.3869884
GGGAGAT2252.6396812E-522.555457
GGTAGGG1650.002594636821.9695957
AGACTGT2053.654197E-421.2194146
GAGTGAT2104.2802165E-420.7281671
CATACTG1750.003650019620.714195
GGGCAGA3201.9059682E-518.1371461
AGGACTG2450.001212929417.7550185
TGTACAG2550.001585792217.0587445
GGGGTGA2700.00231336616.1219081
TCAGCAT2900.00374137714.9999293
TCAAGAC3000.004686955414.4999323
CAAGACC3050.00523045814.2622294
GTATATA3609.568966E-414.1066681
Produced by FastQC (version 0.11.5)