FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGTGGCTGGGCATTCCAGGCGGCAGGTGAGGAAAAAAAGCTGAAAAG11.2658227848101267No Hit
GTCTGTCCCCAGCAATTTTTTTTTTTTTTTGATTTTTAATTTTGCTATTT11.2658227848101267No Hit
CCCCAAGTAAATGCAATTTTGAGAAAAGGGAGTAATTAATTTTCTTTTTG11.2658227848101267No Hit
GGTGACAAGCGAGAAACTCTGACTCAAAAAAACAAAAAACAAACAAAAAA11.2658227848101267No Hit
GGGCTGTGGTGCCTGGTGCGCACACCCTGACCGTTTAATAGAAATTGTTA11.2658227848101267No Hit
CTTCTGAATTGTACATTTTTTGATTAGAGATCCACATATAGTCTCATGGA11.2658227848101267No Hit
CTGTTGAGTTGCTGGGAACGGTGAAAATAAAAGTGTGACTTGATTGGTGG11.2658227848101267No Hit
TGTCAAGTCTGCTATGTGTAAAGGAACGTTGATCTCTGTGAGTGGAATAA11.2658227848101267No Hit
CGGCGTGGTGGTGCACGCCGGTAGTCCCAGCTACGCAGGAGGCTGTGGCC11.2658227848101267No Hit
GGGTGTGGCTTTCCGGAGGGGGAGCCTTGGTGGTCAGGTGGAGGAGGCAG11.2658227848101267No Hit
TATTTTGCTGTTAGGATGATTTCCTAACAGCAAAATATGAGACTTTTAAA11.2658227848101267No Hit
CATGGAGGCAATTGATTTAAAATGATTCCTGACCCTCAAAGATAATAAGA11.2658227848101267No Hit
CTAATTTTCTAAAAATCTTAACACATTCTGAACTGGTTAAAGAGCCATGA11.2658227848101267No Hit
CCAAAAAACAATGTGTACGTCACCATCATCAAATACAAAACATAGATGAA11.2658227848101267No Hit
GGGCAATGGCACAATATTGGCTCACTGCACCCTCACGCCGACTGGGTTAA11.2658227848101267No Hit
AATTATCCCTCATTATAAAAGAAACAGAAAAAAAGATCGTTAAGTGACAC11.2658227848101267No Hit
GGGGGCATCCCGGCTAGGACCAGGGCCAGCTGTCCTATCGCATTCTCTGT11.2658227848101267No Hit
CTCGATGCAATATTTTCAGAATCAATCGCTGTGTTTCTATGTCAGCACGG11.2658227848101267No Hit
CGATAAGCCGGTCTCATACAGCTCGTTCTCAGTCAACACGTACCCACGAC11.2658227848101267No Hit
CATAAATTATTCAGTGTGTGTAAGTCGGATTTGTCTAATCTATGTTATTA11.2658227848101267No Hit
ATACAGTAGTGAGCGTGACATGGTCCCTGGTTTCGTAGACCCGACACTGC11.2658227848101267No Hit
GGCACAAGAGTTTGGTAAAATATTTGTCAAAACTTATGTAAAATAGTTCA11.2658227848101267No Hit
ATTTTATGGAATGCAATGGAATGGAGTGGAACAGAAAGGAATGGAATAGA11.2658227848101267No Hit
GCCGGGGGCTGAGTGCCAAAAGACCAAATAAACCAAATCGAGCCAAGCCA11.2658227848101267No Hit
TTTCAAACCTGCTCTATGAAAGGCAATGTTCAACTCTGTGCCTTGAATGC11.2658227848101267No Hit
GTGGCGGGCGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAAT11.2658227848101267No Hit
ATACAAAGAACTATGTTATGAAAAGGTGAAAGTCTTTCTCACCTTGTTTT11.2658227848101267No Hit
GAGGTGGGTGGATCCCTTGAGGGCAGGAGTTGGAGACCAGCCTGACCCAC11.2658227848101267No Hit
GTCCGGCTGTCCTTTGAAGCCAGGCATTGCCCTCTCCACCCGATGTAGAA11.2658227848101267No Hit
ATACAGAAAATTATTTACCAATATTACTATTGTTGGATGTTTGTGCCCTT11.2658227848101267No Hit
GGGTAAAATCATGAGCCCACTAATTTTTCTCACGTACTAAGAGTGAGAAA11.2658227848101267No Hit
GGTTGATATTTAGGGGAAGTGACAAGTACGCTGCTCTTAAACTCTGAAGG11.2658227848101267No Hit
GGGGGCAATTCTCCCTGGGCCTAAATACCTGTCACAAGGTGGCTTAACAC11.2658227848101267No Hit
GTCCCTGCGGAGGCTCCTGGGTGGCCCAAAGGGATGAGCGCGCAGAAGGG11.2658227848101267No Hit
GGGGTAAGAGGGAGCATCAATGGATTGTTGAGAGCTTATAACCCACTAGA11.2658227848101267No Hit
ATTCACATCTAAATATTATGCAGCCAACAAAAAATTATGGAGATCTATAT11.2658227848101267No Hit
GTATTTCGGTGCAACTTAAACTGTAGAAATCAAAAAAACAACTCCACCCG11.2658227848101267No Hit
GAGTAGCCCTGCCTCCTAAGTAGAGAAAATCTACCTACTGGGCACTGTGC11.2658227848101267No Hit
GGCTGTAAAGTCTGCAAGCAGATATTTGGCCCTCTTTGCGGCCTTCGTTG11.2658227848101267No Hit
GGGTTAGGAGGTAGGGCCTTTGGGAAGTGATTAGGATATATGGGTGGATC11.2658227848101267No Hit
CCACCAGCCTTCTCCCAGAGCCTCTGATTCATGGGCATGTTATTTGGGGA11.2658227848101267No Hit
CTGTTTGAACAGTTGGGCACCTGTGATTTGCCCACACCCAGTGATTGGCT11.2658227848101267No Hit
GCCCCCTCTCCTTCAGGGAGTGGGCTATGCTATCTCCCTCTCAAGGCTGC11.2658227848101267No Hit
GGGCGATATGATATTCCCCTGAATGCATCGGCCCCACTTTATTCCGCGAG11.2658227848101267No Hit
GGGGGGGGCTGTACCCTGCAACTCCACAGGGGCCGCGCTTTCCTAGGCCA11.2658227848101267No Hit
TTTCAGTACAATGTAAATAGTTACTGGAGTGTGGTATGGAAGAGCATCAT11.2658227848101267No Hit
CTATGAAAGGGAATGCTCAAATCTGTGACTTGAATGCAGACATCACAAAG11.2658227848101267No Hit
GCGTGGGATAGCCCCAGGTCGTGCCTGAAGCTGCTATGGTTAGACGACAC11.2658227848101267No Hit
CTTTGGGAGGGTGAGACGGGAGGATAACCTGCGGTCAGGAGTTTGAGACT11.2658227848101267No Hit
CATCACGACACTCAACAGGTACATTGAGCTGGCCCTCAGAAAACTGGAGC11.2658227848101267No Hit
GGGGTGGGTGTAGCTTAACATCAAGCCCCCTGCTTACAGCTGGGAGATTT11.2658227848101267No Hit
AAGGTGCTGGGATTACAGGCGTGAGACACCGTGCCTGTGCTCAAAGGGAA11.2658227848101267No Hit
GCCCCAGCACCCACCTACCAGCTCTCCCGTGCATGTGCCAAGTGCCTGTG11.2658227848101267No Hit
TGAGAGGATAAATAGGTTATTTCAACCTGTTAGTAGGTCGATGGTAGAAT11.2658227848101267No Hit
GTCTGAGATTATAGGTGTGAGTCACCTTGCCTTGCAAAATCTTCAAGTTT11.2658227848101267No Hit
GCGGTCGGGTCGGGTTCCTGATCTCGATGGGAATGCAATCAGGGTGTCAC11.2658227848101267No Hit
GGGTAAGCCAACGTTGGGTAGTATCAAACATATGTTGAGGGGCTGGAATT11.2658227848101267No Hit
GTCTCAAAAAACAAAACAAAACAAAACAAAGCAAAGAAACCCGAATCTAT11.2658227848101267No Hit
GTCTACTCCTGTGGTTGCTGTCCTCCAGTTGGAGCTCTTGAAGCTGGCAG11.2658227848101267No Hit
GGGGGGGGGGGGTTGGGCTCAGGATATATTTTGAAGGGAGAGTCCAAAAG11.2658227848101267No Hit
GTGTTGGGATTACAGGCGTGAGCCACGGCGCCCGGCCTGCCGGCTGTATT11.2658227848101267No Hit
CGTGGATTGTATTGTTTTGTATTTTTTTCAGTCAACGTCTCACTCTTGAG11.2658227848101267No Hit
GGACTACAGGTGTGAAGCACAATGCGCGGATACCGTGTGTAGTTTTTGTA11.2658227848101267No Hit
ACCACAATGAGATACCATCTCACACCAGTTAGAATGGCGATCATTAAACA11.2658227848101267No Hit
TAGTGCCTCCATATAGAACATGAAGGACTAGAATAAAGTAGAAGTATAAT11.2658227848101267No Hit
ACAAAAACAGTTAGTTGTTTTCCTTTAAGTAATAGGTTTGTGTTGTTCAT11.2658227848101267No Hit
GTGTGCAGGGACCAAGTATTAGTATGCCATATTCTTTTGGTATATACATG11.2658227848101267No Hit
GGGGTTATGTGAACTACAATCCAGGATGAGAGTTGGGTCACGACGAAGCC11.2658227848101267No Hit
ATGCTAGGCAGGTTGGAATGTGTAACCGTTACAAACAGGCAGAGGTTAAA11.2658227848101267No Hit
CTCAGAAACTGAGTTGTGATGTTTGCATTCAACTCACAGCGTTCAACATT11.2658227848101267No Hit
GTGGGTAGGGAGGGAAGTGCTGGCTGGTGACTGAAACTGGCAGTGGTTTT11.2658227848101267No Hit
GCCCCGCGCTGACCCTGCGCTGGCAGGTAGGGTTCTGTGAATGGCTGCAC11.2658227848101267No Hit
TAGAGAGCTTCATAGCATGTGCTTCTGCGAAGCTGGGACTAGGCGGTGTG11.2658227848101267No Hit
TCACTGCAGACTCGACCGCCTGGGTTCCAGTGATACTCCTGACTCAGCCT11.2658227848101267No Hit
GAGCAAGACTCCACCTCAAAAATAAAAAATAAAACCAAACAACCCAAAAC11.2658227848101267No Hit
ACTTGGGCACTATGGCCATTTTCATGATATTGATTCTTCCTATCCATGAG11.2658227848101267No Hit
GTGTATATTTCATTATGAGGGAAGTTAAAACATTTCTATTTCTTTTTATG11.2658227848101267No Hit
ACATGTAAGAAGGCAGGATAATGGGCATGTAGGACAAACATCTATAAGTG11.2658227848101267No Hit
GGCGCGATCTTGGATAACTGTAACCTCTGCTGCACAGGTTCAAATTATTA11.2658227848101267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT50.0145.04
GCAAGAC50.0145.03
CTCCACC50.0145.09
ACTCCAC50.0145.08
CAGAGGC50.0145.0145
GAGCAAG50.0145.01
AAGACTC50.0145.05
AGACTCC50.0145.06
AGCAAGA50.0145.02
GACTCCA50.0145.07
Produced by FastQC (version 0.11.5)