FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACCTCCACATAGTGATCATTCTTAAGTACAGAGTATAGATATGCA30.18808777429467086No Hit
GGCCTGGTCCCAAAACCCCACCTAGGCCGGGCTTCCTCTTCCTCCCACAG30.18808777429467086No Hit
GTGCTCCAGTAACAAACAACTTGAAAATCTCAACAGCTTAAAAGGCCAAG30.18808777429467086No Hit
ACAGAAGCATTCTGAGAATCACGTTTGTGATGTGGGTACTCAACTAACAG30.18808777429467086No Hit
CACGCTGAGTTCATCACTGCCAAGCAGAGCCACATGTAAGTTAGGCTGAG20.12539184952978058No Hit
CCACTGCAATATCCATATCCACAGTTGATAGTCAAGACCCCTCATTTCCC20.12539184952978058No Hit
TATTAGTACTTATTATCTAAGATTATGATGAAGATGAAAAATAGTTATGT20.12539184952978058No Hit
GGAGGAGAAACAGGGGCTTTAAAGACATTTTAAAAAAGAGTTTAGAAGGG20.12539184952978058No Hit
GTGCAGTGGAATATTTACCAGAGAGACAATCACAAAGTTCACTGTAATGG20.12539184952978058No Hit
TAGTAATGCATTAACTTGTACATTTATGTTTTATGCACTTCTCTGCAATA20.12539184952978058No Hit
AATCTAAACCTAATATAATGCTTGGAAAAAGCCAGGTGTGCTTCCTGTCT20.12539184952978058No Hit
TCCTCATATTTTGTCCATGCCTTTTTTTAATCTGTCTTTGCTGTTGTTGT20.12539184952978058No Hit
CCTTAGACCCTTATTTTACCTTCTCTCCCTGATGACATGCTTAACTGGTA20.12539184952978058No Hit
TCTGAATATATTACAAAGCTACAGTAAGGCCAGGTGCAGTGGCTCACACC20.12539184952978058No Hit
GTGTGCCCATGGGCAATAACCTAGCCCGAGTCTTCATCTGTAAATAGGGC20.12539184952978058No Hit
TCATCATAGAGATCTTTCACTTCTTTGGTTAAGTTAATTCCTAGGTATTT20.12539184952978058No Hit
GTCTAATTGTTATATGAAGATATTTCCTTTTTCACCATAGGCCTCAAAAT20.12539184952978058No Hit
ACCCTAGAAGCAATCCTAGGCAATACCATTGAGGACATAGGTATGGGCAA20.12539184952978058No Hit
CCCGAGTAGCTGGGACTGCAGGCGCCCGCCACCAAGCCCGTTTTTTGTAT20.12539184952978058No Hit
ATAATGATCTAAATGCCCCCACTTAAAAGGCACAGAGTACCAAGTTTGAT20.12539184952978058No Hit
TCTCCTTAAGCTGATAAATAACTTCAGCAAAGTCTCAGGATACAAAATCA20.12539184952978058No Hit
TAATACCAAGGTGAAACTTACAAACTAATCCTTCATTTATAGCCTATTCT20.12539184952978058No Hit
CCTCTGCACTGCACTAGTAGAGGTTCTCCATGAGGGCTCAAACCCTGCAC20.12539184952978058No Hit
CTTTTCCACTCTCTTGCCTTTTAGTGGTTCTGAGGGATTAGGCTACATAT20.12539184952978058No Hit
ATTTCTTGCCATCCACTCAAAATGAGAGACTAGAAGAGGTGACTACATGT20.12539184952978058No Hit
CCACTTTAGACAGGGTAATCCTGTCCATTACCCAGTTCCAAAGTTGCTTC20.12539184952978058No Hit
CTTTTAAGGCTGTCTTATGTTTTCAAAGAACTTTTTAAGAGATAGGGGGT20.12539184952978058No Hit
AATTTGGACAAAAAAGGTGACACCTTCCAGAAAGGAAGTTTCTTACATCA20.12539184952978058No Hit
TCCTTAAACCACAGATACTTGGAACAATGAGCTGAGAGCAAGAAAGGAAG20.12539184952978058No Hit
TGGTACCACTGTAGTGGCAGTGACAAAGGGGCTGTCAGTTGCTTCTGGGA20.12539184952978058No Hit
TTTTAAAGCCTCCCTTGTGGGTTTGTCAGCTTCAGGGTTCTAGTTATGAT20.12539184952978058No Hit
TGACAAAAATGATTTGCTAGATTAAATGTCTAAGGTATTTTTAAACTATA20.12539184952978058No Hit
CCTATACCCTTTACACAAAGGAGACAGACATTTGGAAGTTTATATTCTGT20.12539184952978058No Hit
GATTCTGAGAATGATTCTGTCTAGTTTTTATACGAAGATATTTCCTTTTC20.12539184952978058No Hit
ATATTATGCAATTGGTACTTTTTTAACTAAGCAAATTTTATAAAGAATTT20.12539184952978058No Hit
GCCCAGATACTGGTTTTTAAATATTACTCTCCACTAAAAGGAGCCAGGTC20.12539184952978058No Hit
GATTACTGCAACCAGTGGCCTCTCTTGGACTTTGCTGAGAGAGTGAAGTA20.12539184952978058No Hit
ATACAATGAGAAACAAAAATATTCAAAATCCAATTAGTACATGCAACTTC20.12539184952978058No Hit
GTGGAGGTAAATTCAAGAGGTATTTTGGAAACTCAGCCAAACAGATGACT20.12539184952978058No Hit
CTCCAAATGTGCTTCTGGCTAGTAGCAGCTATGTTTATATGAATTCACTC20.12539184952978058No Hit
CTTCCTACCTTCTTTTTTTCAGGGGAGTTCTTGGTAGACAAGAAAGAGGG20.12539184952978058No Hit
GCTCTGCACAGCTCCGGGCAATGCCTGGCTGCTTTTCCTCGCAAATTCAG20.12539184952978058No Hit
GGTTATGTTAGGGTTGTACGGTAGAACTGCTATTATTCATCCTATGTGGG20.12539184952978058No Hit
GATGTAAACAATCACAAGAGATTATGATATAAGAATTCCAGGACACACAT20.12539184952978058No Hit
GTGTTAGTTGAGGACACACATCAGAAACTAGTTTCTGAGAATGCTTCTGT20.12539184952978058No Hit
GTCATAGATAGCTCTTATTATTTTGAGATACGTCCCATCAATACCTAATT20.12539184952978058No Hit
GCTCACTGGCTTGCACAGAATGAAGAATAATAGCCTTTTTGAAATGTCAC20.12539184952978058No Hit
CCCTCAGTCTCTCTCTTCTATATTCTGGAAGGTATCTTTGACCTTATCTT20.12539184952978058No Hit
GGATTGGACTCAAATAGAATTATCGAATGGGCTCGAATGGATTCATTGAA20.12539184952978058No Hit
CTATGAGTCTTTGTTACCTGTTTATCATAAAATTTGGAAATCTGAATCAC20.12539184952978058No Hit
TCTCTGTCTTCTTCAGTTCTGCTCTGATCTTAATTATTTCTTGTCTTCTG20.12539184952978058No Hit
TCCCAGCACTTTGGGAGGCCGAGGCGGGCAGATCATGAGGTCAGGAGATT20.12539184952978058No Hit
CCCCAAGACACATAATTGTCAGATTCACCAAGGTTGCAATGAAGGAAAAA20.12539184952978058No Hit
CTTATGTTTAAAAGGGAAGTAGAGTATAAAAGTTCAGAAAATTTGCAGCC20.12539184952978058No Hit
GTAGAAGGTAAATGGGGAGCAGGCATGCCACATGCGAGAGAGGGGTTAAT20.12539184952978058No Hit
AGCCTGGCCAACATGGTGAAATCCCGTCTCTACTCAAAATACAAAATATT20.12539184952978058No Hit
ATATATACCTTGGGTACTTTTATATTTTATGTACAGTATATAATATATAG20.12539184952978058No Hit
ATCTTTGAGGAAACTTAGGATTTGATGATGATCTCAGGGTAGCTTCATGG20.12539184952978058No Hit
GCACCTAGCAGGTTTTGATGAATAAATACACTGGACACGTAAGACTTTGA20.12539184952978058No Hit
GGAAGTGGGGCAGGGGACAGAAAAAGAATGAGGAAGGAGGGACAGAGAAA20.12539184952978058No Hit
GAGATATTCCTTTTTATTTGAGGTTTCAAGCTTCAATATCTTGAGTTCAT20.12539184952978058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA400.007246867318.12560-64
Produced by FastQC (version 0.11.5)