FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_14_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_14_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223425
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGC300.001933792172.499778
AGGCGAT350.003562923862.1426666
GGCGATG400.006034134454.3991851
ACGCATA450.00962953348.3331838
TAAGACG450.00962953348.3331834
GGTATTC951.0263481E-438.1577768
GCCTACA1450.001220294225.011121
GGGGCTA1550.001799452823.3974971
GGGCAGT1750.003640573220.7234971
ATGACTT2104.2997912E-420.7142225
AACAGTC2155.03936E-420.2324967
CTCCAAG2556.902964E-519.910811
GGGGTGT2801.4149828E-418.133061
GTGTGGA2000.00789749618.1249439
ACAGTCC2050.00910503317.6828738
CCAATAT2650.002051079416.4150453
AATACAG2750.002626541515.8181335
CTAATAT2800.002961823715.5356664
GGAGTGC3507.7713624E-414.4999545
GAGGAGA3100.005826061614.0322146
Produced by FastQC (version 0.11.5)