FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127509
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAG259.368141E-486.99935
GTGTGAT1157.0371025E-637.8703351
CTATCCT800.002030050636.2497024
GGGGTAG950.004685116530.5620231
TCCTGCA950.00471219230.526072
GGGTAGG1050.00763992227.6513561
GCTCTAT1050.00763992227.6513561
GGCTTAT1100.00958575726.3944761
GGGCAAC1658.295848E-526.3944761
CCTACAG1100.00964095926.3634223
CAAGGCT1100.00964095926.3634224
GAACTGT1450.001220639124.9997986
GGGGCAG1550.001787675823.4144541
ACACTCT2202.2092656E-523.0679959
GGATTAT1600.00215015722.682751
CTATTAT1650.002571021721.9953961
GGGCAGG1750.003616890420.7385161
AGCAGTT1750.003641669420.7141177
CCAGGCC1900.00586063319.0787948
AAGTTTC1900.00586063319.0787947
Produced by FastQC (version 0.11.5)