FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237661
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAG1352.1324771E-532.2286261
GGTCTAG1306.4607855E-427.8901621
TTTAGAC2156.092214E-726.976433
CTAATGC1958.718647E-626.025343
GGGGATA1450.001222275125.004971
GGGTAGA1450.001223775924.9997082
AGCACCT1801.5156524E-424.1663885
TTAGACA2451.9020754E-623.6731934
ATATGTG2552.692961E-622.7448355
GTATATG1952.606826E-422.3074343
CTAACAC1650.00259525921.9694423
TGCTAGA1800.00429786520.1386572
TTACACA2556.9296155E-519.9017314
TAGACAG3751.7939601E-719.3331095
GGTCTGA1900.005868224419.082741
GGCGTGA1900.005875365319.0787268
GTGTATA2307.9269585E-418.9168031
CAGTGTA1950.006826046418.5895279
TATATGT3151.6749153E-518.4124854
TACACAG2400.001056562218.124795
Produced by FastQC (version 0.11.5)