FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTCAGCCCCCTAAAAAGCAGTTGCCTGCAAAGGCTCAAAAATCCAT12.1739130434782608No Hit
CTGTTAACTACCTCTGTTTAACTAACAGAAATTGATGGAGGCTTCAAGGC12.1739130434782608No Hit
CTTTTAGACTACCAATCCGATACATTCCCATGTTTAGTCAGGTCCCTGGC12.1739130434782608No Hit
GGAGGGGCTCGATTTCTTGCCCTAGTGATCAACCTCCCTCAGCCACCCAA12.1739130434782608No Hit
ATATAAAGCCTATCTGGCTTAAATGCAGGATTGAAAATCTGCATTTAAGC12.1739130434782608No Hit
GGTTGATAGTTTTAATATTGTAAAAATATTTGCCAATATATTGTTGGATG12.1739130434782608No Hit
CCAAGCAGATGGATCTCCGTGCCAACCGGCAGTGGCAAGTGAATACAAAG12.1739130434782608No Hit
ATATTTTTCCTTTAGTTCAACGCAATCCCATCAGGTTTGTAATGTTCTTA12.1739130434782608No Hit
GCCCCCAAATCATTTGCGATTTAAGTACAACAAAGCCTAGCTATTTCTAC12.1739130434782608No Hit
CCGGGTTACAGCTGAATGCAAGAAAATTTTATAAGCAACTGTTATCATCC12.1739130434782608No Hit
CAGTAATGGAATTGCTGGGTCACATGGTAGTTCTGTGTTGTTTGAGCAAT12.1739130434782608No Hit
GGATAATATATGCCACAGAAACTGGCAGATGCTACAAAACAGAACTTTTC12.1739130434782608No Hit
GGATTACGTTCATTGCTTTGCATATGTTTAACAAGACTGACATCACTGGG12.1739130434782608No Hit
CAACTCTAAAGGGTAATGTACACTGGCTAACTATTAGCGTCTAGGGATAA12.1739130434782608No Hit
GGTACATACCCACCATGGGATACTATGCAGCCATAAAAAGGAACAAGATC12.1739130434782608No Hit
ACAAAAATAACTCAAGATGGATTAAAAACTTAAACATAAGCCCTAACATT12.1739130434782608No Hit
CTGCCCTCCAGCCTGGGCGACAGAGCGAGCATCCGACTCAAAAAAAAAAA12.1739130434782608No Hit
GTCCCTCCCACAGCACATGGGTATTACCGGAGCTACAATTCAAAATGAGA12.1739130434782608No Hit
GGCTTTAAACCGCTCACAAATATCCCTCTGCAGATACCACAAGAAGACTG12.1739130434782608No Hit
TTCTGAAACCTGGATTTCACTACATGAAAGAATTATCATTTAATTTTTCC12.1739130434782608No Hit
TTTTCCGGTTGATTTTCACACATTGTGACAGTTGAGAACCACTGGTGTAG12.1739130434782608No Hit
ATTAGGAACACGAGCAGTAAGTTAAAATAAACAGAGCGGTATCAGTATGA12.1739130434782608No Hit
GGTGCCGGGTGGGACGGTCATTGCCGGTCAATTGGATGGCTTTTGCTTCA12.1739130434782608No Hit
TTCCTCCGGTACCATGATTTGCTAGAGCGACTAAAATGGTGAATAAACTA12.1739130434782608No Hit
CCTTAAATCATATTAACTTTTTAGGAATTGAAAGTAAATAAATTTAACAA12.1739130434782608No Hit
GCCGTCCAATGCTGGCTACAGAAGAGACAAGAACCTATTTTCCTTTTTTG12.1739130434782608No Hit
CTTTATACCTCTAGGAGATTATTATGGAACATTTCACTATGGACAGAAGA12.1739130434782608No Hit
CCTATTTACATTATAAATTACCAAGTCTTCTGCAGTTCTTTATAGAAGTG12.1739130434782608No Hit
AATTACAACAGTAAGATTGGTTTAGTTTAACCAAACAAAGAGAAAGTTTA12.1739130434782608No Hit
AATAAAGATTGAGTACGTGCTATGTGACAGCCTAAAATCTTAGTAAGCCA12.1739130434782608No Hit
ACTATATAGATTTGAATTAACCAAAAAAATAGAATCATAAGTGAAAAACA12.1739130434782608No Hit
TCCCAGGAGGTGGGGACCCCTGAAATACACATTTTCAATCGCATCTATCT12.1739130434782608No Hit
ACAGAAGTTAAGCCTTCAAACAGACATTGTTGGTGCTGGTTGAAGTTATG12.1739130434782608No Hit
GAGGCCTCCCCATAAATCTGGAAGTGTACGTTACATTCAACTGCGGTTTA12.1739130434782608No Hit
CCGGGGAGCAGGAGGAGGAGCATCTTCATGTGGGGTACCCTGCAAATGCA12.1739130434782608No Hit
CTCCCAAGAGTAGCTACTATCCTGAGTTCTAACAGTATAAATCAGTTTTA12.1739130434782608No Hit
AGGGAGTGGAATGGCCTCAAATGCAAGTAAACCGAGTGGAATCGAAACAA12.1739130434782608No Hit
AGGTGTGGTGACAAAGACCCCGGTGAAGGCACACTACAGCAGGTGCAAGC12.1739130434782608No Hit
CCCTTGTGCTATTTGCAATTAATATGTGCTTCAAAATCAAGCCCCAAGCT12.1739130434782608No Hit
GGTTTTTAAGATTGTGGGAGAATTAAACGCATATTTAAATAGATGTTCAC12.1739130434782608No Hit
GCATAAATGAGATTTGTGACATATATCTGCACCGATCACTTAATTTATGT12.1739130434782608No Hit
CCCTGACAAGGAAATTATAGGTAAGTTTTGAAGAAAACCAGATACAATTA12.1739130434782608No Hit
GAATGGAATCGAATGGATTCATTGAATGGAATCAGATGGCATCATCGTAT12.1739130434782608No Hit
CACAAATGTTTTGTAAAGCATTAATGACTTCACAATTCTTCCACCCATGC12.1739130434782608No Hit
ATTATATGGGCCGTCGGTGATATTACAAGGTTTTGAAAATTATTCCCAAT12.1739130434782608No Hit
CCTCTGTCTGCTGGGTTAAAGTAATTCTCCTGCCGCAGCCTCATGAGTAG12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers