FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_135_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_135_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300131
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT800.002034383436.2498746
GGACGGT1051.8506715E-434.5236936
CCCTAGT1255.130416E-429.0144021
CGCACAC1450.001224467624.9999143
GGCCTAC1500.001487533424.1786671
GGGGGTA1851.8203742E-423.5251921
TATACCA1851.8265563E-423.5134325
GGGCTAC1550.001800344223.3987121
GTAGGGC1650.002596711721.9696223
GGGGTAA1700.003078928521.334121
ATAGTGC2053.658276E-421.219448
GTCCTAT1800.00428782120.1488911
GGCGTAT1800.00428782120.1488911
GTCCTGG2908.182729E-620.0099331
TAGACAG2908.218183E-619.9999315
GGCGTGC1850.00502435519.6043261
TAGCACA1850.005038907319.5945264
TACACTG2659.322186E-519.1508795
GGGGTAG1950.00681012518.5989741
GGTTGAT2000.00788091218.1340031
Produced by FastQC (version 0.11.5)