FastQCFastQC Report
Mon 28 Jan 2019
HGYGMBGX9_n01_S_116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYGMBGX9_n01_S_116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287242
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTT657.3028816E-444.646261
GGGGTAC900.003611698732.2445221
CCCTTAG1504.3672673E-529.0200711
CTCCGGA1000.00606748928.9998632
GGGTACA2101.5417736E-524.1833921
GTCTAAG1500.001491727824.1665522
GGACCCT1550.001805416523.3869846
GTGTAGC1600.002162686822.6719281
GTACAGC1700.003075123821.3382871
GTGTAGG1750.003637855220.7286221
GAACCAT2155.0442433E-420.2324626
TATATGG1800.00429984420.1387922
CCTATAT2556.935696E-519.9018653
GTGCTAG2256.8195333E-419.3467121
GCCGAGG1950.006801753318.6026081
ACTGATC1950.00682916418.5896538
GTCTTAG2050.00907478417.6951661
GATATAT3352.8298862E-517.3254171
ACTGAGT2600.001807552216.730698
CACAGGC2600.001807552216.730697
Produced by FastQC (version 0.11.5)