FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57005557
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9076751.5922570496065849No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1239200.2173823159029917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC18900.023.4485871
CCGATCT19950.022.1873262
TCCGATT18000.019.2864631
ACGAGCC16450.014.082814145
CCGACGC21000.013.789423145
GTATATA118350.012.1076741
CCGATTT24600.011.9955872
CGATCTA12203.45608E-1111.4599483
GTATTAG54800.011.4568611
GTGTATA78150.011.4362511
TCTATAC47250.011.2129413
GTCCTAG33600.011.2113551
TATACTG64350.011.1433135
AGGGCGC27350.010.852553145
GTATAGG37450.010.8477121
CTAGGAC31050.010.4274943
TACCCGC16700.010.403996145
CTCCCGC43750.010.259331145
CTATACT55150.010.2449074
GTACTAG22750.010.0648561
Produced by FastQC (version 0.11.5)