FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAGGGGAACTGGTCCTTTACTAGGATTTCTTTCTCATCATAGCTCC19.090909090909092No Hit
GTTCAAGAAAATCAGCACTCAGGCATCCACAACACAGCGAGAATCATATA19.090909090909092No Hit
GTTCTAGGCCAAACAAAACTGAAGACCACTGTGCTTGGAAAACAGTTGTG19.090909090909092No Hit
CAAAAACATCAATAAGGAATATAATAAAAGAAATGTTTATAGATTAAAAA19.090909090909092No Hit
GTTTAAAAAGCTCACCTCAATTCCACATTTCTGAAGTTGAAGTGTTACTA19.090909090909092No Hit
CCCTGCAAGCTCGACACCGTGCACCTGACAGCTATGACAAAAATAGGTTT19.090909090909092No Hit
ACAGCAAATACACCAAACTGGTTATTAGTTTCTTATAAGTCTATGTCATT19.090909090909092No Hit
GAATGGAATGGAATGGAATGCAATGGAATGGAATCATCCGGAATGGAATG19.090909090909092No Hit
CTGTTCCACTAAAGAGAACTCCTAAAAATTTTTATTTGAAAATATGGTAT19.090909090909092No Hit
AATTTCTTTTTAGCACCAAATAACATTTTATTGTTTGGATGTACTACAGT19.090909090909092No Hit
GGTTATACCAGTGTTTTTTTTTTTTTTTTTTCGGGAAATTAACACTATGA19.090909090909092No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers