FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_76_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_76_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15307565
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG64550.016.19511
GTATAGA99150.015.1563641
TATACTG133850.013.5912255
GTATATA246200.013.4165471
GTGTATA168850.013.2854061
GTCCTAG75600.013.059741
CTAATAC103050.012.9441173
TATACAG134350.012.7853895
TACACCG23300.012.7535535
TCTATAC92500.012.5395183
GTATTAG102750.012.2231091
GTCTTAG92100.012.2177011
GTATACG17000.011.9571191
GGACCGT17800.011.807986
CTATACT100250.011.7846054
TAATACT143350.011.7806714
GTCCTAC53850.011.5939161
GTGTAGG78350.011.582131
CCTATAC61150.011.4994713
TACACTG134750.011.402775
Produced by FastQC (version 0.11.5)