FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATGGAGTGGAGTTGAGTGGAGTGGAGTGGAGTGGAGTGGAGTGCAGT10.6578947368421052No Hit
CTTCCCACTAGAGAAAACACAGGAGAAATATCTCTGGCAGATCCCCCACC10.6578947368421052No Hit
CCTCAACATAATCTTATTACAGTGAAGCTTCCCTCTTTAAAGTAAATCTT10.6578947368421052No Hit
AGTATTCATTATGTTATGCTCCAGTATTATGACATTGCAGTATTTTCGAA10.6578947368421052No Hit
GGGGGCTGGGGGGGGGGGGGTGGTGGGGGGGGGAGGGGGGCGGGGGGGGG10.6578947368421052No Hit
GCGGGGGAGGGGTGGGTCGGCCGGTGGGGGGGTGCCGGTCCGGGGCGGGG10.6578947368421052No Hit
CACTTAGGCTACACTACATTTATTTTAAAAATATTTAGGTCTGGTGTGGT10.6578947368421052No Hit
GTGATCTGTTCGAATAAAAATGCGGGGGTGGGGGCTGAAGAGGAGGAAAC10.6578947368421052No Hit
GTGCTATATCTGTCTAGTCTTGCCTACTGAAAGCAATGCAATTATGGCTT10.6578947368421052No Hit
CTTCCCTGCATGGCCAGTGTGGGCGGCGGAGAGCAATTTTCAGTCTTTTC10.6578947368421052No Hit
AAGCAGTGGCTACCAAGAGGTGAAAGTGGTCCAGTCACAGCAAAAGCAGA10.6578947368421052No Hit
TAGAGAACCTGCAGGCAAAGCCAGGATTTTTCTGAAGGAACAATTCTGTG10.6578947368421052No Hit
CTGTGACCCAGAGGCAGACATAGAGCGCTGGTGGAACTCTCTTGAGCCAC10.6578947368421052No Hit
TGTTAACCTGTCTAAAAACATTCAAATTCATGTGTTTTCTTTCTTTCCTT10.6578947368421052No Hit
GACTCGGGGTGCCAGTGTTGGGGGGCTAGTGAGATTGGAGTTAGGGGTGT10.6578947368421052No Hit
GACCTCCTTGAGGCCTTCGTTGGAAACGGGATTTCTTCATATTCTGCTAG10.6578947368421052No Hit
CCAACAAGAATGATCGCTAACATGCATTGTATTGCCACCCCGGGGGGTTC10.6578947368421052No Hit
TTCCACAATATAGACCATTACCATGATCAACCGAATTGATATTTCACTAC10.6578947368421052No Hit
ATGTAACAATTTATTTTATTGTGGTCAAAAACACATAACATGAAATTTAC10.6578947368421052No Hit
NNNTAAACTCTTTCATATGACATTCACATTTTGGTCGACATGACCTGTGG10.6578947368421052No Hit
TGGTGGTGCATATAGCAACATGTCATCCTTTTTCAGTGTCTTTTTAATTT10.6578947368421052No Hit
GATCTTAAAATCTGCGCCAATGTACTGTTAAATATCTGATAGTGTTGTAT10.6578947368421052No Hit
ACAATAGACTTTGGAGATTCAGAAGGAGAAGAGTGGGAAGTAGGGTGTGA10.6578947368421052No Hit
GGAGTGGACTGAAGAACAGTACCTGTGATGATGTCACCAATGCGGCCACG10.6578947368421052No Hit
ATACACAAGATTACTGTGTGTGTTTGTATAAAATTTTCGAAGTTGCATCA10.6578947368421052No Hit
CCTTTAACATTAAATGCACAACATTCGTACCACTTAAAACTGGGGTCAGG10.6578947368421052No Hit
GCACAAACGGTAGCCACTTTCTATTTCCCCCCTTTTGTCAACCTTGAGCC10.6578947368421052No Hit
ATTCCATTTGATGATGACTCCATCCGATGCCATTCGATGATTCCATTTGA10.6578947368421052No Hit
GACTTGAATTGAATGGAATCATCAAATGGACTCGAATGGAATCATCATAG10.6578947368421052No Hit
GAAACACTACTGATAATGCGTAGGTTGAAGTCACGTTTCAGGTCAATCGT10.6578947368421052No Hit
TGTATGTACCGCATTTTGTTTATCCATTCATCTGCCAATGGGCATTTGGG10.6578947368421052No Hit
GTATGAAAATGGAATAGTTACCCTCTGAGTATCTAGTAACAAATCTTTGA10.6578947368421052No Hit
GAATCATGGAACGGAATTGAATGGTATCTTCATCGAATGAATTGAATGCA10.6578947368421052No Hit
TTCTTCATCTAATGAGGATAAAATTATGTTTAATAAGAGACTAGGAATTC10.6578947368421052No Hit
GCCCACGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCAC10.6578947368421052No Hit
ATGCTTTTCTGTCCTTTTACTGCACCTCTTCCAGACCTATGGCATTTGGG10.6578947368421052No Hit
GCACTAGAGCCACGTTGCTAAACTACAGCTGGATTCTTTTTTGTCTTTTT10.6578947368421052No Hit
ACTTTAGACTGGGCGCGGTGGCTCACTCCTGTAATCCCAGCACTTCAGGA10.6578947368421052No Hit
CTATTACTCATTAGTTATTTTTTCTGATCTTTTCCCTCCTCCCACCCTCT10.6578947368421052No Hit
AAAAGACTCTAGTACATAATTTACTATCTAATAAAAATATAGTACTAATT10.6578947368421052No Hit
GGTTCATATCTGTTCAAATAAAAACTCCTCATTAAATATATGAAGAATCA10.6578947368421052No Hit
ATTTATGAGAGATAAGATAAAAAACATTGGCGTATCCAACCTGCAGAGTG10.6578947368421052No Hit
CTCCTAGTCCCCCTTCCTAGAGGAACCTTTTCAAATTATTTTAAATTTTT10.6578947368421052No Hit
GCTTGACAGGAGGCGTGTTAGGCTAGTGATCAAATATAGGGATTTTGCTG10.6578947368421052No Hit
GTCGTACAGTGGATATCTTAAACTTACTCCTTCTAAATGAAATTTTATAT10.6578947368421052No Hit
CCTCCTGCCACACGCAGGTGACAAACACCCAGATGACCCACCTGGCCCCT10.6578947368421052No Hit
ACCCTGCCAGACCATTCGGAAAACTTTCCAATACGTATATAATCATGGAA10.6578947368421052No Hit
AGCTTTTACAAAAGCAGCAGGAAGTACTTACTGAATCAGCACTGGTCCAT10.6578947368421052No Hit
GCAGGGAGAGAGGAATCAGATAAGTCTTCTAAAACTGGATTGTGATGAAA10.6578947368421052No Hit
CCACCATATCCAGCTAATTTTTTTTTGTATTTTTATTAGAGACTGGGTTT10.6578947368421052No Hit
TCCCACCTCAGCCTCCCAGAGTGCTGGGATTACGGCCAGTTTACATTCGG10.6578947368421052No Hit
GCCCAGGCTGGTCTCAAACTCCTGACCTCAAGCAATCCTTCTGTCTCAGC10.6578947368421052No Hit
TCACTCTTCACCTCTACCCCCTTTAGTCTGGCTAACTCCTCACCCTTCAG10.6578947368421052No Hit
CTCTTGAAACTTCTGTGTTAGTTTTGTCCTCTGGAAAGGAGATGCTGAAT10.6578947368421052No Hit
CCCCTGATATGCTGCACTGAGCAGGCCACATCATTAAGGTCGTATTATTG10.6578947368421052No Hit
AAGTGATACTATCCTGAGGCTATCTCATGCCTTGCAGCAAATACTTATTA10.6578947368421052No Hit
CAAAAGGATGTTGAAATTTCCACAAATAGTTGGCGTATTTGCCAGCTTCT10.6578947368421052No Hit
AATTAGAACTCAGGTCTCCTGGCTGCAAAGCTGATGCTCCCCACAACAGC10.6578947368421052No Hit
TACATATTGAGGATTTATATCACCTAGACTCAATCCAGTGTAGAACCAAG10.6578947368421052No Hit
ATATTAGTCAATTTTCATGCTGCCAATAAAGACATACCTGAGATGGGTCA10.6578947368421052No Hit
CTCCCGCCCCGGGCCGCCGGTCGGCCACGCAGGGCGGCGGGCGGGGGGCG10.6578947368421052No Hit
GTATTTACGATGTAGTGGATAAGGGGCAAGATAAACGCCCCGTTATAAGA10.6578947368421052No Hit
NNTTAGAGCTTGCGCCCTGGATAATCTGTGTCGCGTTACTCTGGGCATAT10.6578947368421052No Hit
ACCCTGGGGGAGGAGCCCTCAGTATAGTTGGGTAACCAAAGCAGGGTTTG10.6578947368421052No Hit
CCTATAAAATATTTTACAAAGCCCTATACATACGGTCACATGTACCAGCA10.6578947368421052No Hit
CCTTAGACGGCTGTCTTGTGGGTTCTGTGGCACCTGGGTTCTGTCTGGGC10.6578947368421052No Hit
AGAGTTAAGATCACATAGTTTGACAAAAATAAAAACAAAATAATGAAAAA10.6578947368421052No Hit
TTAAAGAAAATCATACTCACTAAATGAAACAGATTTGGTTTTAACAAACT10.6578947368421052No Hit
GAAATAAGATTTACCAAATGTTCAAAAATAATTATGATGAATCACAATCG10.6578947368421052No Hit
GCTTAGAGACTGATGAGGGATGGGTTTGAACCGCAGAAGGGTGAGAGCGA10.6578947368421052No Hit
AAGAAAGGTTCAACACTGTGAAATGAATGCACTCATCACAAAGAAGTTTC10.6578947368421052No Hit
CTTCCTTCCTTTCTTTCCTTCCTTCTTTTTTTTTTTTTTTTTTTTGTTTG10.6578947368421052No Hit
CAACAAAACAGTACTAGTCTCATTCAAATATAATATATAAAACACAGATA10.6578947368421052No Hit
CTTCTGCTCAGAGGCGGCCTCAGTCTCTTGATTTAGAACTTTTCAAATGT10.6578947368421052No Hit
GCCTGGGGGGTGGAGGTGGAGGTGAGTGGTCCTAAGTGAAAGGGCTGAGG10.6578947368421052No Hit
GAAATGGTTTTCTATTCATCGGCTTCTGGATACTTAGCAATTTTCCTTTT10.6578947368421052No Hit
AGGTGTCCGAGACTTGTTCATAGTTATCTATTAAATAGAGGACGTGTGGT10.6578947368421052No Hit
GGGCTGGGGACAGTGGCTCACGCCTGCAATCCCAGCCCGTTGGGAGGCCG10.6578947368421052No Hit
CCTAAAAAGCACAAGCAACAAAAGCAAAAATAAACAAATAGGACTATATC10.6578947368421052No Hit
ATTCGATTCCATTCGATTGCACTCGGGTTGATTCCTTTCCATTGCATTCC10.6578947368421052No Hit
CTGCTACATTCTCCGTCATTTTCTCTAAAATTGCCCTGATACTTTTCCTT10.6578947368421052No Hit
GTATTCTCGGATGAAAAGGGGGTTAATTAGTTTACATTATGATCTGTCAC10.6578947368421052No Hit
AGTTTTTAATTGCTGTCTATAAAGCCAAGGCTATTAATTCATCAGAAGAC10.6578947368421052No Hit
TTTTTGAAATTACATAGACATACAAAGACAAAGTGAATTTCACTACAGCA10.6578947368421052No Hit
CTTCTTGTCTGATGGGGAAAGCCACTGCTTCCTCATTTAAGCAGCAAGCC10.6578947368421052No Hit
ATCAAATTGACATAAAACTATCACAGTGAGTATGTATACCTATGCACCCT10.6578947368421052No Hit
TTCTTGAAGAGAGCATACCTCTGCAGCTGACATAGAAACCTCCATGTGGC10.6578947368421052No Hit
GATTAGCAGCTTGGAAGGGAAGAGGACCGGCCAAACCAGGAGTGGGTAGA10.6578947368421052No Hit
ATATAAAATATGTCTCGTAGTAATGTTGGTAATTAATAGGCAAAATTTAG10.6578947368421052No Hit
CATCCAGATACTACAGAAAGTGTGTTTCAAACCTGCTCTATGAAAGGGAA10.6578947368421052No Hit
GGAAAGCCCCTTGGCATGAGGAATTCCAGAGATGCAGATTGTAAGTCAGG10.6578947368421052No Hit
CACTTCCTATGTAACGTGAGTCCTTACACCCTAGGGCGTGCTCAATAACC10.6578947368421052No Hit
GTATTCCTCGTAACTGTCACATAAAGGAAGTCACTCAGGACTACCAAGGA10.6578947368421052No Hit
GAATGCAACTGATAGGAAACCAACCAGTCCAACCACACTGTGCCACCTAG10.6578947368421052No Hit
ACATAGGCATGGGCAAGGACTTCATGTCTAAAACACCAAAAGCAATGGTA10.6578947368421052No Hit
GTGGAGTGCAGAGGAGTGGAGTAGAGTGGAATTGATTGGAGTGGAGTGGA10.6578947368421052No Hit
GATTAAGCATCAGGACTTTATTAAGTTAACAACAATTGCTTGTGGAATTT10.6578947368421052No Hit
GTCATTAATCAATACTCAACATCTCATATAGACATTCCAAGCCATAATAT10.6578947368421052No Hit
AATTTATACTACTGCAAAGACAGTTATTATCAAGATGTACATTTCCCCAG10.6578947368421052No Hit
CCTTATAACCTATCTTTGTTTGCAATAGGCCTTATCAAATATGTGTAGAA10.6578947368421052No Hit
ATATCTCATTGTGGTTTTGATTTGCATTTCTCTAATGACCAGTGATGATG10.6578947368421052No Hit
CAATAATTCAATTTTAAATTTTTTTAATTAAGGTGGGTACTATTGAACAA10.6578947368421052No Hit
GGGGGGGGGGGGGGGTGGGGGGTGGGGGGTGTGTAGGGGGGGGGGGGGGG10.6578947368421052No Hit
GGTAATGAGGTTTGATAATCTGCAGAGACCAGGGGTGCGACCCGGCATTG10.6578947368421052No Hit
TCAATGGACACATAAGAAATCAAAAGAAATTAAAATGTTTCCCTCAGAGC10.6578947368421052No Hit
GTGGTGGCACGCACCTGTAGTCCCAGCTACTCGAGAGGCTCAGGCAGAAG10.6578947368421052No Hit
GCATAAATGCACAGCACGTACAATTATATACAATACATAATACTGGATAA10.6578947368421052No Hit
CTTTTTGGGTGTGTAATCTGAGGGTTTTGTATAGATTATTATTGTGTTTA10.6578947368421052No Hit
AGAAAATATAGCCTATATACACAATGGAATACTATTCAGCCATTAAAAAG10.6578947368421052No Hit
GCTAAATTATTGAGACTTTTCACCAAACAGAATATAATAGAATTATAGTG10.6578947368421052No Hit
CATCGCCTCTCAGCTGCTCATGCACCTGCTCCCTTGGCCTCCTGTGAAAA10.6578947368421052No Hit
GCGGGTGGGGGGGGCGGTGGGGCTGGGGGGGGTGGGGGGGGGTGGGGGTG10.6578947368421052No Hit
GTAAATAAAAAATAGATTTTTAGAACTAGTAATACTTTTACATTTTTGTT10.6578947368421052No Hit
AGTTAAAAATAGAAAATTTGACCCGCTCTCCGCGCAGTCTAATTTGGTTA10.6578947368421052No Hit
ACCTCTCACTCCTACAACCGCGAGATTTCTGTAATATTTACTTGTAAGTT10.6578947368421052No Hit
ATGTGACTGCAGTCGTTGGCTCCCAAGACAGAGAAGAAATCTTACTCTGT10.6578947368421052No Hit
TTCTTAATCCAGTCTATCATTGTTGGATATTTGGGTTGGTTCCAAGTCTT10.6578947368421052No Hit
CAAATATAAAGAGGATCTGTCAGTGAACAGAATCCAGAAAAGGTTAATTT10.6578947368421052No Hit
GTGTATTTTATGTGTGACCCAAGACAATTCTTCTTCCAAGTGGCCCAGGG10.6578947368421052No Hit
ACCAAGAGCCTGGGGCGGGCGGGGGGGGGGGGGCGGGGGGGGGGTTGAAC10.6578947368421052No Hit
GAGAGAAGGAAAGACAAAGACAGACAGACAAAGAGACAGACAGAGAAAGA10.6578947368421052No Hit
GGCAAAGATAAACAGGCATGGAAGCATAAAATACACTTTGACTAAAACCT10.6578947368421052No Hit
CCAGCTACTCGGGAGGCTGAGGTATGAGAATCGCCTGAACCTTGGGGGGC10.6578947368421052No Hit
GTGATATTGTTCTTAATATCCAGGGGTGGAAAGAATGATATTACTCCCAA10.6578947368421052No Hit
CTCTCTCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAACCGGTAGGTG10.6578947368421052No Hit
ATTGAGAGGAGAATGGGCCAGAGGCACAGGGAATGTGCCCCTTAAATGCT10.6578947368421052No Hit
CATCAGGGAAGTAAAAAGTAAAAGCAAAATGAACTAAGACCCAGAAGAGT10.6578947368421052No Hit
ATGAAGAAAATGTGGTGAAACCATCCAAAGGTAAATTAGGTAACAGAAAA10.6578947368421052No Hit
GTGCTGAAGTGTGGGTGGCAAGGGAGGGAGTTGAGAGAGAAAGGATAGAC10.6578947368421052No Hit
ATAGAAAAGCAATATGGGGTTCTGGCTGTAAAGCACCTGAGCTTTTTTGA10.6578947368421052No Hit
ATTCCATTCGGGTCCGTTCGAAGATTCTCTTCGATTCCGTTCGATAATTC10.6578947368421052No Hit
CTCACGATGATTCTGTTTTCGTATAAGTGTCGTACCTGATGTGTCTGCTT10.6578947368421052No Hit
GGTTAGGGAAGATAATATAAGAAAAGATTTAGGATTGAATCACATGACAT10.6578947368421052No Hit
GGGCTGTACTGCTGTCACAATCCCAATCTCTTCATTTTGCAGTAATCAAC10.6578947368421052No Hit
GCGCAGAGAAACCCATGTTGAACATCCCCTCCCTCACTGTAGACCCTTTT10.6578947368421052No Hit
AGTGGGGCCCTGGGATCACACTGACCTGCGTACAAATCTCAGTTTGCCAA10.6578947368421052No Hit
AGGATGCAAAGGCATAAGAATGATATAATGGAGTTTGGGGACTCAGTAGA10.6578947368421052No Hit
GGGACATTCCGGGCAAGGATTATGCTATTCCTTCGGACTGCCAACTGTAT10.6578947368421052No Hit
TTTCAGGTCTGTGAACTAGATATCATCTTTAATTTTGAGAAGGCTTATTT10.6578947368421052No Hit
GATGTGTGTACTCTTAGTTTATGGACAAAGTATTACTAAAAATTCTCTTT10.6578947368421052No Hit
GTGTTCTATTGTGCTTGGGTTCTTTCACTTGACAGTATATTTATGACATC10.6578947368421052No Hit
CTGTGAGACTATGGATAAAAAAAAAAAAAACAAAAAAACAACAAAAAGAA10.6578947368421052No Hit
GTCTGGGCTGGTCCCTCTGGTAACTGTCTTATGGGTCTTCTCCCTCCACA10.6578947368421052No Hit
GTCCTGGCTGCTGAGAAGCTAGACGACTCAGAATGATGGGGGTTGACGCC10.6578947368421052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGTGGTGGGGGGGGGGGGGGGGGTGGT10.6578947368421052No Hit
ACAGGGGGGGAGTGGGGCTGGAAGGGTGGCATTTTGTGGGCTGGATGATG10.6578947368421052No Hit
CCCTGTGATAATTTAATGCATTCTATTTTTAATTTAAGAATAAAAGACGA10.6578947368421052No Hit
GCCCGCCCAACCACATGAGGGGGATCGGGATCGGCCCCCTGCGGTCGGGA10.6578947368421052No Hit
AATTTATAAGTTTAGTATCGTGTTAATTAAGTTATTTGTATTAATCAAGA10.6578947368421052No Hit
CTTTTGGAGTTACTGTCTAGTGTCAGGAGCAGATTGAGTGATAAAATGCA10.6578947368421052No Hit
GCTAACATCATCATGACAGAATCAAATTCACATATAACAATATTAACCTT10.6578947368421052No Hit
CCCCTCACAGGTGCGCCAGAACTACCACCAGGACTCAGCGGCCAACATCA10.6578947368421052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA450.005476619616.11111320-24
Produced by FastQC (version 0.11.5)