FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_70_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_70_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17999485
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG84700.015.2548311
GTATATA295000.014.4685891
TATACCG33650.014.430045
GTATAAG122950.014.110431
GTATAGA123500.013.9300361
GTATTAG134850.013.7265091
TATACTG160450.013.68613155
GTGTAAG116600.012.8867281
TACACTG140900.012.4989385
GTATAAT185300.011.9480021
CTATACT122700.011.8726434
GTGTATA189500.011.8364131
GTGTAGG97100.011.81161
GTATTAT215800.011.7393711
TACACCG45000.011.434675
ATAATAC207750.011.2351363
GTCCTAG75200.011.1972411
GTATTGG136350.011.1798251
GTATATG183600.011.0702191
GTCTATA106100.010.9465051
Produced by FastQC (version 0.11.5)