FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18524496
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG176450.016.100755
GTATAGG75250.014.6565761
GTATTAG137650.014.65426351
GTATAGA126450.014.2307661
GTGTATA227650.014.151771
GTATATA331550.013.8750851
GTATAAG125300.013.55065251
CCTATAC74050.013.2137693
TCTATAC122050.013.1835763
GTACTAG63450.012.8080141
TATACAG175250.012.6545045
GTGTAGG95750.012.5795391
GTATTAT213700.012.4611171
GTGTTAG151650.012.3923241
CTATACT131950.012.0836264
TAGACTG141700.011.8658775
TACACTG172350.011.7320695
GTATATG197300.011.73164751
CTAATAC129100.011.5653553
GTATTAA189350.011.4578021
Produced by FastQC (version 0.11.5)