FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_50_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_50_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973383
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG5407.4578566E-1118.7899045
CTTACAC5107.0940587E-1018.4750563
GTATAAG5751.8007995E-1017.6697711
ATAGGCG2901.8701644E-417.494055
TTACACT7750.016.8329814
GTATAGA5904.834874E-915.99049951
GGACAGT5503.592322E-815.8128026
ACTGTCC4601.5426613E-615.7555098
TACACCC5655.000038E-815.3929925
GTCTAGG3355.5565557E-415.1643541
GTCTAAT4852.6387352E-614.9633541
TCTAGGG3406.25425E-414.9298312
ACCGTGC6801.9917934E-914.9213948
GTCTTAA5908.3649866E-814.7604611
ATAGGAC5003.668678E-614.4958123
GTATTAG7053.254172E-914.4115151
GTATATC5607.5759453E-714.2369593
CTTAGAC4101.60906E-414.1422573
CTATAGT5658.380357E-714.1102434
GTATTAT9303.6379788E-1214.0462471
Produced by FastQC (version 0.11.5)