FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCCTCTCTGACAGACTGTCTCAGAGTCGGCAAAAGAATAAAAATACTG11.9607843137254901No Hit
ATCTATATAGTCATACATTTATACGCGGATAGGGATTCCCTAGGCTAATT11.9607843137254901No Hit
CATTTCACCTTATTGTGGAAACAATGCAAGGAATTAGGCGGTAATTCTTA11.9607843137254901No Hit
TAGCTGTTATTGGAAGCAAAAAATAAACAAAAATTGGCATATAAGTATGC11.9607843137254901No Hit
GTTAAGCTGTTCTATGAGGCGTTATAACTAAAGCGATATATCAACCGGTT11.9607843137254901No Hit
GGGAGCATACGAGCGCACATGTAGGGAATGCTAAGCTGAGGCAGGCATTG11.9607843137254901No Hit
GCTATTTTTCGTACCGAATGATTGGGTGGAATCGTTAGTCGACTTAGCTC11.9607843137254901No Hit
GTGCAAACGTCAACGCTGGGGGTTGCCTCTGACCCACCAGATAACGTACC11.9607843137254901No Hit
GAAAATGGTGACCGATTTACGGCTTAATTGGATCTTGTATTATATTTAGT11.9607843137254901No Hit
AAAAAGGTCAGACCTGTATAAATGATAAGAAATATGAAGCAAAAGTACAT11.9607843137254901No Hit
TTACAACTTGCTTGTGACATTCTGCTAAACATTCAACACAAGGGTCTATT11.9607843137254901No Hit
CACAAAAAATTACATAGGTCAATGGTTTATCTCTTTGGATTAAACAGAAA11.9607843137254901No Hit
GACCACATACAGAGAGTCACTCGCTGACAATGTGACCCAGCCACGTTCCT11.9607843137254901No Hit
GATACCTTCAAGCAAAAGACATTCTACAAAATCGTCCACTCGTAAAGCGG11.9607843137254901No Hit
CTAATTATAATATGTACCGAGTCGCGAAGATTGTCTCTTCAAATGAATTC11.9607843137254901No Hit
CAGAACGATTGATCAGTGTAATGTTAGAGGAAAACAAAGACCTGAGAAGA11.9607843137254901No Hit
TACACAGACTGATACACAAATCCCTCGAGTATCTTTTTATAAACTCAAAA11.9607843137254901No Hit
TACTTGTTATTATTTGAAGTTTTAGTTTTTAGTTGGTAGTTGAATTAGGG11.9607843137254901No Hit
GCAAAAAAGTATACCTCTTAAATTTTATAGAAGTCACTTTTTCAACGTTT11.9607843137254901No Hit
AAAGACATTTTGATAATAAGTGTACTAGGACTGCACTTTAGAGCCTATTC11.9607843137254901No Hit
GGACGTAAGTCTTGCTCATTGCCTTTTGTACCATTAATAGTAAGACCTAG11.9607843137254901No Hit
GCCATTAATCTTAGATAGGCTAATAGCAAACAAGTCGTGACTCAATAAAT11.9607843137254901No Hit
CCTAACAACTAATCCTATCAAGTCGATTGGAACTAAGGAAAATTAACCTA11.9607843137254901No Hit
AATTTTTACCGAAACACAGACCCCTGGATGTCTAACCAGTGAGATTTTTT11.9607843137254901No Hit
TGTATACATACAAAAATTTATGGAATGTATTAAAGCACAAATCATTCAAT11.9607843137254901No Hit
AATCAATAGCTAACAAAAAAAATACAACTCATTAACAAAGAAAATTTATT11.9607843137254901No Hit
NATTGCATTCCTTTGCTATGCAATCCATTCAAACCGTTAGGAAAGTTCAG11.9607843137254901No Hit
TACGCGAGAATTATAAATAAGACAACCTACTTCATGATTTCTACTTATCC11.9607843137254901No Hit
AAATAACCTAAAAGAGAATAAAATTCCTTTTTAAAGAGAAGGTTTTATAA11.9607843137254901No Hit
GCTACAATATTTTTGTAACAGACTCAACTACATTTATAAAAAAGCATTAA11.9607843137254901No Hit
CTTCGAAAGAGTAACTTAGGACTAAAGGATATTATGTGGTATAAAAATCT11.9607843137254901No Hit
GGATAGTTTTTCACCTCAGAGAAAGGATTGGTAACATAACTGTACTCTGG11.9607843137254901No Hit
CCCTTAGTTATCAGTAGTTCTTTGATGTGTATTATCGCATAATAATAATA11.9607843137254901No Hit
GCACTGGAATACTGGTGATTGCTCAGCTAAGGAGCAAGAGCATGCGTAAT11.9607843137254901No Hit
AAGTATATGACCTTCTCGTCATGGGACTTGCATGCACGTAACTAACTTTT11.9607843137254901No Hit
CGCTGAAATTTTTAGGAATACTCTTACATCAAAAAGAACAGTTAACGTTT11.9607843137254901No Hit
CATTTCATGATGATTCCTTTGAATTCCATTTGATGACAATTCCATTCAAT11.9607843137254901No Hit
GTACAACTGATTGCATTAAGTCTAGCAACCTGATTTGGATCCAGTCCCAT11.9607843137254901No Hit
GTCTCAACCAAGATACAAGACAACTGACATCACAGACTCAAACGAAAGAC11.9607843137254901No Hit
GTCATTGAAATTCGAGGAGTAGTACAGTATTTGACATGCGCATCGTCATC11.9607843137254901No Hit
TATTTATATGGTGTCCTAAGAACTTTTAGGGTTATAGTCTCCTAGAATGC11.9607843137254901No Hit
CTGATGTAGATCTACTAGTGATAGGTCGAGACAGTGTTTTTGTCTGGCTT11.9607843137254901No Hit
CCAATGCCTCCTGTGTGTAAATAATTTTACATAACGTCTACCATCTCCCA11.9607843137254901No Hit
ATTTCGTAAAGAATCCTAATTACTTAACGACACCTATGCTATCATTTTGT11.9607843137254901No Hit
GGAACCTAATCTCAAAATATTACTTTTTATTCAGATAACAAAGTATATTT11.9607843137254901No Hit
GATTTTAGGTAGCAGATTATAAATTCTTGGCATTCTAGTCATGCGCCAGT11.9607843137254901No Hit
TACCAGGATTTTATAAAAATATTTAATTTATTGGAGGGTAGAGAGGCGAA11.9607843137254901No Hit
GTCTTATTTCTTCGATTGAACGTTACATTCGTTGTTTCGAAATGTAGTCT11.9607843137254901No Hit
CTTTAAGGTTATATGTTTGTGAATAACACTAAGTGTCCGGTATTTTAGTC11.9607843137254901No Hit
TTTAAATACTTGGAATATTACGTTTTCAAATTCTGTTGGGTGACCCATGG11.9607843137254901No Hit
CCTATGTGTAGCAAGGCGAGCGTCCACTGCAAATTTGCGCGTCTTCATCG11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTTCA50.0145.07
TTTTCAC50.0145.08
GTTTTTC50.0145.06
TTTCACC50.0145.09
AGGCGGA50.0145.0145
TAGTTTT50.0145.04
GGATAGT50.0145.01
ATAGTTT50.0145.03
AGTTTTT50.0145.05
GATAGTT50.0145.02
Produced by FastQC (version 0.11.5)