FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_38_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_38_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16948084
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG72700.015.7778331
TATACTG147200.014.4752755
GTATTAG118450.014.09671
GTATAGA111300.013.8934181
GTATATA290800.013.8555341
GTGTATA200900.013.7318281
TACACCG15700.012.925455
GTATAAG105450.012.7364911
GTACGGA18850.012.7094651
TATACAG155950.012.6406915
GTATATG178750.012.6310371
TCTATAC105650.012.4193143
CTTACAC118500.012.4184173
GTATTAT182350.012.2622351
CCTACAC86100.012.2082493
CTATACT112700.012.1544534
GTCCTAG89000.011.9909061
GTCTAAG91350.011.9208551
CTAGGAC92050.011.8916393
TACACTG161600.011.839955
Produced by FastQC (version 0.11.5)