FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_32_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_32_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631901
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA150200.016.8702351
CCTATAC43050.015.9973143
TATACTG90000.015.7837225
GTATTAG77800.015.3982021
GTATAGA72700.015.0802391
GTATTAT97700.014.4912461
TAATACT94000.013.95601754
GTATTAA94950.013.9168871
CTAATAC78300.013.6098163
GTATAAT91900.012.9566881
TATAATA133950.012.9416322
GTATAAA135050.012.6877061
GTGTATA123800.012.6090821
GTATAGG46300.012.5451151
ATAATAC96200.012.5092053
TATTATA134500.012.4572912
TATATAC151350.012.453393
GTATTAC52350.012.2048281
TTATACT102300.012.1860684
GTATAAG72100.012.1847261
Produced by FastQC (version 0.11.5)