FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATCTAAGAGACTTTAATCTCAACAAATATTATGCTTGAATCATTTTAA11.9607843137254901No Hit
CCTGAGAACAAGCAATCCTCACAACTTGCACTCTAAAAGTTATGGTATTA11.9607843137254901No Hit
ATATTAAACTGTTACTGAATTAATTTTGCAGGCGTATATTATCTAGATCT11.9607843137254901No Hit
GTCTTAAGGGAGTGCTAACTATGGGGAAAAACACTGGTACATGCCACTAT11.9607843137254901No Hit
GTGCTGGTTCAAGTCTGTCATATCAAATTTTCCACATTTGTAAACTGAAA11.9607843137254901No Hit
TTGTCTGTGTGTCTGTTTTTTTAAACTTAAAAGAAGCATTATTTATCAAA11.9607843137254901No Hit
CGGTTACAGCAACGACACTATTTGCCACGAATAGGGCCGCAATAATAAAT11.9607843137254901No Hit
GGCAGGGACCGGCTACGCTCCTTCGCCGACACCCGGTCAGCCAAATCAAA11.9607843137254901No Hit
CGATCGGGGACAGAGAATGACTGCCCAGCTGACTTGGCATGCATCATCAT11.9607843137254901No Hit
GGATAAAAAATCAATGTACCAAAATCACAAGCATTCTTATACACCAACAA11.9607843137254901No Hit
GTATATTATATGTATCACAGCATCATTGTGTGCGCAAAGAATATGTTTGA11.9607843137254901No Hit
GTCTCATAAATCAAATGTACTAAAACATAAAGAACACACAAAAAAAACCA11.9607843137254901No Hit
GGACATAGCGCTCCCACGGAGGGTGGGGTGGGGTTGGCAGGAACCCTCCA11.9607843137254901No Hit
NAGCACAGAAACCATTACATTTTGCACAAAAGTAGTTTTAAGAAGACACA11.9607843137254901No Hit
GATAAAGAATAAAGGAGATAATAGCTGTCCAAGCAATGACAGTCAGGCAC11.9607843137254901No Hit
CTACAACCCAGGCTCTGGGAGCAAGGTCACCCTGTCGAATTCCGCGCTCC11.9607843137254901No Hit
TCCTTAAAAATTTTGTAAATTCATACTCTGTATGTTTTTCAAATTGTAAC11.9607843137254901No Hit
NAAATGGACTTTCTGGAGTGGAAAACCAGCTAAGGTGTCTCCGTAGAATG11.9607843137254901No Hit
CAACATACACAGAAGTCAATACAGACGACCCACGAGCCATTGAATATGAT11.9607843137254901No Hit
AGGGGATCCGGGATTATTGCCCAGATATGCTGGAAACAAAGGCTCAGCGC11.9607843137254901No Hit
GTATGTATGTTTGTGTGTATGTATGTTTTTAGGTATGTTAGTAATGATTG11.9607843137254901No Hit
GCAACGGACCTTTGCCTACCATGGGATCCTCCACATATATACTATTCAAG11.9607843137254901No Hit
CTACTAGACAAAAAAACAAAAAAAAAATAAGTAATAATTTAATTAGTAAT11.9607843137254901No Hit
GGCTTTGCACTCATACAAACAGGAAGCTGAAATTTTGAGTTTTGATGGTC11.9607843137254901No Hit
CAAAACTCGCGTCTGTAGAAAAACAAAAAGAAAAAGACAGACCTGATCAG11.9607843137254901No Hit
AAATCTCAGAGTGAGAATAAAGACGTAAGTTCTGACTCTAAGAATTGGGA11.9607843137254901No Hit
GTGTTCACCGTGTGACTGGCATGCAAACATCAGAAAGCAGTTGCTCAGAA11.9607843137254901No Hit
ACATTACCATAAACCAGCACCCATATGCGTAGATGTACACAAAATAAGAG11.9607843137254901No Hit
TTACTTTCAGAGTTGTCCCTGATAACAGTATCATACATTTTTGTTGTTTA11.9607843137254901No Hit
CCTTTGAACAACACAAAATTCAATGTAACAATATAAGATAAAATCAAACA11.9607843137254901No Hit
GCTAAATGTTCCCAAACACTAAAAAAGCAAGAGATGAATGCTCATTTATA11.9607843137254901No Hit
TGGATACACATTGCGAAATCTTAATGGGAATAAATTAAAGTTATGAGAAA11.9607843137254901No Hit
CACTTGACAAAATCTACGAAAGATAGAGAATATGATAATAGATTTGTATG11.9607843137254901No Hit
GTTTATATTTGTATCTTTTGTAAAGCAGATAGAACTTTTATAATAATTAA11.9607843137254901No Hit
ATATCTTGTTTTTTGTTCTTTAGTACTCCTGCCGGGGTAGACAAGAGGTA11.9607843137254901No Hit
GTCCTGGTTGGTTGCGCATAAATAAAAGAACAAATGCTCTAATTTCTAAG11.9607843137254901No Hit
TGAAAAGTCTTCTTATTTAAGCCCTCTTTCGCCTTCTTGTCAAACAGAAG11.9607843137254901No Hit
CTGATTACTAATTGCTCAAGACCTATGTGGGTTTTTGTGTGAAGGTGATT11.9607843137254901No Hit
GTTTTCTAAAGGAGGAAGATCAGAAGATAAGTAGCTGTATTAGACTGGGT11.9607843137254901No Hit
GTTGAGTATCTAGTATGAAGAGGATACTGGAATAGGCAAACAGCAAGGAA11.9607843137254901No Hit
GACAAACATGCGTGAGAAACTTTTAATGTAAAAATAATATTTTTTTAACA11.9607843137254901No Hit
AATTTGAATCTTCTTAATTTTATTTTGGGAGTACTTCAAGAAATGCAAAC11.9607843137254901No Hit
TGGTATAACTGAGGTTCATGAAGATTAAGGAATTTACCCAAGGTTCACAC11.9607843137254901No Hit
ATACTACACTGCCCCGGTCGCCCCCATATGTGGTTTCTAGCTCGTGCTGG11.9607843137254901No Hit
GGTTATTTTTGATTCCATTTGATGATGATTACATTCGATTCCATTTGATC11.9607843137254901No Hit
ATATTAAGGAATTTGAATACATAGGGGTGACTATTATTTATTTGATATTA11.9607843137254901No Hit
AAAATTACCGCAAGCTGGATGAAAGATTTATGTTTATTTTTTATAGTTAC11.9607843137254901No Hit
GCAACAAACGGAATACGCAAAATTACCAGACTCACACTATTCCGCACACC11.9607843137254901No Hit
GTGATACTGTATTGGGTTCATATATATTTAGGATAGCGAGCTCTTCTTGT11.9607843137254901No Hit
CATATAAATAGCAAAAGAACGGAATTTAAAAAACCTACAAGGATTAAGTA11.9607843137254901No Hit
TATTAAAACAGTCCTGAAATTAGATCACAAATATATCTAATAAGAATAAT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCA50.0145.05
TCAAGTC50.0145.09
TTCAAGT50.0145.08
GCTGGTT50.0145.03
TGCTGGT50.0145.02
GGTTCAA50.0145.06
GTTCAAG50.0145.07
CTGGTTC50.0145.04
ACGACAT50.0145.0145
GTGCTGG50.0145.01
Produced by FastQC (version 0.11.5)