FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_26_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_26_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10702648
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG41200.016.7407841
CTGTCGC33200.014.6259689
GTATAGA64450.014.0810971
GTATATA180050.013.8308971
GTATTAT115600.013.5029921
GTCCTAT45400.013.2730771
GTACTAG33900.012.8499351
GTGTATA119250.012.8461641
TATACAG95350.012.7697545
GTATTAG78100.012.7355861
CTAGGAC50850.012.687923
GTATAAG67250.012.6311531
GTACCGT7501.1575139E-712.5623826
TCTGTCG36750.012.4242758
GTATAAC48350.012.3130821
TATACTG98950.011.938945
GTATAAT109200.011.6349561
GTGTAGG55200.011.5742531
GTACTAA54300.011.4986811
GTATTAC58150.011.2367871
Produced by FastQC (version 0.11.5)