FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12209239
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG213400.017.150375
GTATAGA97050.013.7640561
GGATCGT18250.013.5017738
GTATATA431300.013.41538051
GTATTAG96950.013.1043171
ATACTGT253850.013.1042466
GTCCTAG61650.012.8356421
CCTACAC60800.012.757023
TATACAG135950.012.6874475
TCTATAC101200.012.6066933
CTATACT104150.012.5956024
GTATTAT209000.012.2617541
GTCCTAC38600.012.2250381
ATATACT282850.012.1969264
GATCGTT20350.012.1084699
GTATTAC74050.012.0588041
TTATACT161850.011.9563384
TACACTG125000.011.885585
CCTATAC50850.011.8319453
ACTGTAC198650.011.6744428
Produced by FastQC (version 0.11.5)