FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_208_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_208_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25901029
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG44100.015.7766465
TATACTG175950.015.6934255
GTGTATA233200.015.626451
GTATATA280300.015.0465951
GTATAGG101550.014.65409851
GTATTAG173850.014.155011
GTATTAT214150.014.1013411
GTATAGA154050.014.0894691
GTATAAG158000.013.9210081
GTATTAA200150.013.6732241
TACACTG220800.012.9652255
CTAATAC161550.012.9229953
GTCTAAG139900.012.868241
TCTATAC146150.012.8463153
TATACAG195400.012.83315855
GTATAAT194700.012.7137481
TATATAC281200.012.5800423
GTGTAAG172600.012.5331611
ATTATAC183300.012.338713
TATACCG26400.012.0789955
Produced by FastQC (version 0.11.5)