FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_197_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20416730
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG82100.014.2377951
GTATTAG144500.014.1690671
GTATATA276050.012.9926951
GTATAAG132250.012.9012631
GTATTAA194450.012.7696261
TATACTG158350.012.494845
GTGTATA196650.012.4790861
GTATAGA130700.011.943261
ATTATAC190750.011.7045643
GTATAAT196250.011.6166261
TATACAG171650.011.3578095
GTATATG180600.011.3368231
GTATTAT207750.011.3230641
TATATAC279450.011.3097093
CCTATAC78900.010.9329923
TATACCG25900.010.913175
GTATTAC109850.010.90545751
TAGACTG140400.010.8918445
CTATACT132600.010.8769834
TCTATAC124750.010.866013
Produced by FastQC (version 0.11.5)