FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCTCCGCCTCTCGGG21.9607843137254901No Hit
GGGATAAGACAAGTTGAATGGCACTTGGTCAGATGTAGGGATTTGGTCTT21.9607843137254901No Hit
CCCCGCCCCAGCTCCCCCCTCGAAGCTCTTTTCCTGTAGCTGTGTGTTGT21.9607843137254901No Hit
ATACCACCTTAATGGAATCTTTATATCTCAAAATGGGAAATTAAGGCAGA21.9607843137254901No Hit
CCAGGTAGCTTCTAGAATTTTCGGTCCCTTCCTGATTTCCTTCTACTTGA21.9607843137254901No Hit
AAGTAAGGTTAAATGAGGTCATATGGGTTGGGCCAGAATTCAATATGACT21.9607843137254901No Hit
CAGCTACTGGGGACTGCTGGGGACACCCAACCCCCGAGGGTGAGGTCATA21.9607843137254901No Hit
ACCGAGATGCTCTGACACACCCTCCAAAATTCGCTGAGAGATACACCTAC10.9803921568627451No Hit
AATCAAGGCTAGAGGAAGTGTCTGTTGGATTTATTGAGTTCACTGTAAAA10.9803921568627451No Hit
AAATAGCATTGAATCTATGAATTGCTTTGGGGAATATGGACATTTTAACA10.9803921568627451No Hit
ATATTTCCAAATTCAATTACTAAAAAGGAAGATCTATATCTTCACAATTT10.9803921568627451No Hit
ATTCCATTCGATTCCATTTGATGATTGCATTCTATTTCATTTGATGATGA10.9803921568627451No Hit
AAAAAATACAGACCAAACATCCCTAGTCCAAAAATCCAAAATCCAAAATG10.9803921568627451No Hit
GTGGGAGGCCCAGCCTCCCTCGGTCCAGCCTCCCAGGGCTGCCCGTGTCC10.9803921568627451No Hit
ATAATGACCTTAGACCCTTATTCACATCCTACATAAAAATTAACTCAAAA10.9803921568627451No Hit
GAATATGATCATTCAAAGCCTCTTTAATAAGTTGACCACACCCACGACAA10.9803921568627451No Hit
GTTTGTTACGCTTTTCTTCGTGGATTATTGAAGATGATTCCTCTCCTCGA10.9803921568627451No Hit
CCCTAGTGCACTCAGTCAATACCTTCCTCGCTCCCCTTCTCCCTCCAGGT10.9803921568627451No Hit
GCATCATCCTGATGCCAAAACGTGGCAGAAACACACACACAAAAAAGAAA10.9803921568627451No Hit
GAAAAAGAGACAGTTGCTGATAGGGAATCAGGGCCTTTCCTGGCACACCC10.9803921568627451No Hit
TGTTTTGTGGTACATTTAGAAAGAAAATGAATACTGTGCAAGGTTAAATA10.9803921568627451No Hit
GTTTGATCTTTATTATTGTGTTTGAGGCCATTACAAGTGTCGTTGAGTTA10.9803921568627451No Hit
GCACTGTCTTGTGGCTCTTTCCCTAGCTACCTGCTGACCACAATCTTCCA10.9803921568627451No Hit
TCACTCTTTGGTCAATTTCAGTATATTCAAAGTATCCATGTATCAGGAAC10.9803921568627451No Hit
ATTTTCCACTTGGGTCCCCAAGTAAATCCAGCCACTATTTCCAGACCTAA10.9803921568627451No Hit
GTGGTGTGCACCTGTAGTCCCAGCTACTAGGGAGGCTGAGGCAGGAGAAG10.9803921568627451No Hit
CACCAAGCCTAGTAATGCTGTAGTTCTTGTAGACTCATAGAGGTAACATG10.9803921568627451No Hit
CACCGGACGAGTACCGTTGATATGGACACCCGTTCGCGAGCCCACCACTC10.9803921568627451No Hit
CTCTATGAATTTTTTCTTTGTGGCAACAGGAGATTGTAAGTTTGATTTCA10.9803921568627451No Hit
GATAACATGTCGGGATTGGCATCGTCGACCATCATTTTTTCCATATCTAG10.9803921568627451No Hit
ACAAGTTACACTTTGTCCTACTTAACATAACGTCCTTAGTCACTGCCAAA10.9803921568627451No Hit
GTCAATGAAGATCAAAGCAGCGACCTGGACGGCAGTAATTAGAGCAGCAG10.9803921568627451No Hit
AGCTGCTGATGTTGACCTTATACAAATTCCATTCCATAAAATGTATAAAT10.9803921568627451No Hit
TAAAATGAGAATATATTTATGCTAAGACAGAACAAACATTAAAACCACGC10.9803921568627451No Hit
GTATTCCTTCCTCTTAGATTTTTTTTGGAAGAGTTTGAGGATTGCTAATT10.9803921568627451No Hit
ATGTTGACACAAGAAATATGATAGTTGATACGGTTTTTAATATCCGCAGC10.9803921568627451No Hit
CAACTGCTTTGCTACCTAGATTCTAGCACCGACTCTATAATTTCTTTTTC10.9803921568627451No Hit
GGTTAAAGGTGCAGATCCCCCGTGCAGTAAAAAAAAAAAATCATAAAAAA10.9803921568627451No Hit
GTCCGTTACCATCCGAGGAATCCTGGGACAGCCTGTAACCAGGTGTTTCT10.9803921568627451No Hit
CTCCGTGCATCACCATCGAGTGTAATATAATGGCAGCATTGAATGACATC10.9803921568627451No Hit
GAACAATAAGCTCCGGCCAGAAGGTGAAATCTCTACAGTCAAACCTACTA10.9803921568627451No Hit
GGGATATTTTTCTTATTTGTTTGATTTTAAGTATTTATTGATAATTGTTG10.9803921568627451No Hit
GTGCATGCACCTGTAATCCCAGCTACTTGGGAGTCTGAGGCAGGAGAATC10.9803921568627451No Hit
TTTGAAAATTGACCACATAGTTGGAAGTAAAGCTCTCCTCAGCAAATGTA10.9803921568627451No Hit
ATCACATTGTGCGTAGCCAGTGGAAACACCTAAAACAAGTGAGGATATGA10.9803921568627451No Hit
AGCATAAGTTTCTCGCGCAAATAACAGAGGAAATAGATTCACGACTTCAA10.9803921568627451No Hit
TTATGCAAAGAAAGTCCGCCTTTAAGCAATCAGAGTGAGACACTGGCTAG10.9803921568627451No Hit
AAATTACCCAGTCTCCGGTATTTTGTTATAGCAGCACAAATAGACTAAGA10.9803921568627451No Hit
CATTGAGCCTACGGCAGAAAAAGAAACATCTTCCTATAAAAACTAGACAG10.9803921568627451No Hit
CTTAACCTCTCTGAGCCTCAGCTTCTTTATGGCCTGTGGTCAACTTTTTA10.9803921568627451No Hit
CCCCCATTGCTTAAGAGTAGCCATATCTCCCCCTACTGATCAAAATTCAT10.9803921568627451No Hit
AGGCCACACAGTCGGTAAGTGGTGGGAGCTGGGATTGGAACCCGGCAGCC10.9803921568627451No Hit
CCGTTTAAAAGAAATGAAAGGAGCAAATCTAAACATGAGTTTCAAGCTAT10.9803921568627451No Hit
TGTATGACTAGCGTAAACACATGACGACCGCTTGTTAAACTTTATCAAAT10.9803921568627451No Hit
GATTAGTTCAGATTTGAGTAGTTATTTTGACCATTTAATACATTTAAAAA10.9803921568627451No Hit
TTTCAAGACATTATCGTCAAGAATAGGTGGCCAATCCATACATGTATAAG10.9803921568627451No Hit
CATCACAAAGAAGATTCTGAGAATATTTCTGTCTAGTTTTTATTAGAAGA10.9803921568627451No Hit
GAAAACATGAACAATACTATTAAAAATCTCTCAAATAAACTCTACTATAA10.9803921568627451No Hit
AATCAAGGCTAGAGGAAGTGTCTGTTGTATTTATTGAGTTCACTGTAAAA10.9803921568627451No Hit
GTACACGACAAGGTTCGTCCAGAGCTTACGACTGGTTTTACTTAGATCAG10.9803921568627451No Hit
GATTCAACCCAAAAGGAAACCAAACCAATCCAATCAACCGCAGTGCAATA10.9803921568627451No Hit
GTATATAACTAAAACCATACATATCAATATGAATTTAAGACTAAGTCCTA10.9803921568627451No Hit
GGCTAACACAGTGAAACCCTGTCTCTGCTAAAAATACAAAAAAAAAAAAA10.9803921568627451No Hit
GGTAAACGAGAAGGCCTGAGCCCTCCCCAGTTACCACCTTCGAAAATAGG10.9803921568627451No Hit
GCTCCTGGAGCCCAGGCCCTGGCACGAGAAGACGTGTGGTGACTGTAGTC10.9803921568627451No Hit
TCAGAAATGCTACAAACCAAAGAACTTCATAATTTAAAAACTTTTGTTTG10.9803921568627451No Hit
ATTTTGAAAGGTCAGTAAATTCTCTTTTTATTCTCAAAAACTGTGGTTAT10.9803921568627451No Hit
ATGCTGGGAAGGGGTTCCTTGTTACTGCTAGGTAGAGGTCAGAGTCTAAG10.9803921568627451No Hit
GCCTTGGCCTCATGACTAAGTTTGGAAAAATGATATTTGAGTAAAAGTCA10.9803921568627451No Hit
CACTAGTCAAGTGACCCCCAATTAAGGTTATCTGAGGCGTAGCAAATGAT10.9803921568627451No Hit
ACAGTGTTGCAACTTGTTCGCACTGCTGTGCTCGCTCTCCGTCACTCAGT10.9803921568627451No Hit
GCGAAGGAGAGCGCACGCGACGAACTAACGGCTTGTTGGAAGGAACCTTA10.9803921568627451No Hit
GATATTTTATATAATTGATATTCAGTGTTTCGGTGTCATTATGTAATTGC10.9803921568627451No Hit
GAGGAATATTGCTTGAAAACTATGATGTAAATGAGTGAATGTTATCTGCA10.9803921568627451No Hit
TCCTGCTTAGAATTCAATTAATCTGGTGCATGTGCACACACATACGCACA10.9803921568627451No Hit
ATATCGAAACTCCGAGCAACGGCGACGAGAGAGAACACACGTCTTACAAG10.9803921568627451No Hit
CTGTTATCTACATCTCGCATGAGTTTGGTACTTTTGTTAGGATTGATGAA10.9803921568627451No Hit
ATAAAAAGGAATGAGATCATGTCCTTTGCAGGGACATGGATGAAGCTGGA10.9803921568627451No Hit
TTACACAATATTCTATTAATAATAGTAAAAAAAGTCATATTTTATACATA10.9803921568627451No Hit
TGAATTAACTGTGAACTTGTGGACCTAGGATTAGTCATGGAGAGGAACTT10.9803921568627451No Hit
CTACCTGGAGGGGCAATAGGACTGCAACAGAGAGACACCTGTGAGGGGCA10.9803921568627451No Hit
TTGAATCTCTGTTCATTTGAATGTTTGACAACCTTCCTTTTTTGGCATTG10.9803921568627451No Hit
CATAGATCGCAATACAAAAATCAAGAATAGAGTTATGTCTTTAGATATCG10.9803921568627451No Hit
ATTTAACTGGGAATGTCTAGGAAGTGCATGCCAGGTGGGAAGCACATTAC10.9803921568627451No Hit
AAGAATAATATTTATCATTAAAGTTTTTTTTGGGTGTTTATTAACATCAA10.9803921568627451No Hit
ATCGAATGGAATGTACTGGAGAGTAAGGGAGTTGAATAGAATCAATCCGA10.9803921568627451No Hit
GATTTCTTCATTGAATGCTAGACGGAAGAATTCTCAGTAAATTCTTTGTG10.9803921568627451No Hit
CACTCACTGAGCAGATGGGGTCCTGACTGCCTGAAGCTGGAGCACCCTGC10.9803921568627451No Hit
AAAAAAAAAAAGAACAAGTTTAAAATTATAAAAATGTAAGAAAATAAAAA10.9803921568627451No Hit
GTATATGACTTAGGTTATGGGTATATAGTGGTGCTACTTACCAAGATCAG10.9803921568627451No Hit
TTGGAATCTATAGGTTTTCTCTATAAAGCAGTAGATTTGGGGAAACATTG10.9803921568627451No Hit
GTATAATAATTAATAGCATATTCTCAGGAGTTACACTAAGTTGAAGTCCT10.9803921568627451No Hit
AATAAAATCAGAATGTCTAAGAGGATTAACAATGTAGTGCTCATACATGT10.9803921568627451No Hit
CTTTTAATCGAGCTCTGAGATGCTGCTATACACGAACCAATTGCTGCCAA10.9803921568627451No Hit
CTCATATACCTATGTAACAAACCTGCATGTTCTGCACATATATCCCTTTT10.9803921568627451No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers