FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22031117
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG186600.014.7980425
GTATATA350900.014.7718481
GTATTAG156000.014.7055891
GTATTAA208150.013.5672911
GTATTAT229750.013.49249651
GTATAGA147350.013.2039721
GTGTATA253550.013.1135971
CTAATAC153500.012.5149463
TATATAC354650.011.9576933
GTATAAG140400.011.8927051
TTATACT227400.011.82451254
TATTATA356750.011.6702722
GTATAAT215300.011.6668081
ATATACT259800.011.5215444
TCTATAC142100.011.4783633
ATTATAC207350.011.3973813
ATACTGT247650.011.3547976
GTCTTAT160950.011.1861511
CTATACT148150.011.1051394
TATACAG199500.011.0802075
Produced by FastQC (version 0.11.5)