FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23170413
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185450.014.9679955
GTATAGG92050.014.8300241
GTATAGA148200.013.8658351
GTATTAG162900.013.7735281
GTATATA349200.013.7238991
GTGTATA254650.013.6583951
GTATAAG148000.013.2958281
TACACTG211550.013.0185395
CCTATAC91650.012.3393313
CTATACT150000.011.9830174
GTGTAGG116900.011.8017641
GTCCTAG120400.011.6396161
TCTATAC141750.011.60923
GTATTAT239600.011.5160781
GTATTAA216800.011.4879531
TATACCG19250.011.2948955
CCTACAC114200.011.1723883
TATACAG204550.011.1255595
GTATACA190100.011.1152351
TTATACT231050.011.0418644
Produced by FastQC (version 0.11.5)