FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAAAAGCAAAGTAGTAAAAAAGCCTTGCTTCATGTACTTTAAGATGT15.555555555555555No Hit
TAAGTGTTGTGAAATTTCATATTAAATGCAAAAAAAAGAAACTGGAGTAT15.555555555555555No Hit
GAAGAGGAAATAGGTGTTCGATGTTGATAATACATGATAGAAGCACTGGT15.555555555555555No Hit
GGGTCAGGCTGTTAGACTAAAAGCTGCAGATCTGAGGCTGCTATTCCAGA15.555555555555555No Hit
AACAAGAAGTTAACCAAATGAGAGAGACATAGTATCAGAAAAGGTAGGAT15.555555555555555No Hit
GAAGGGAATCGAAGTGAAGTTAAGGGAATGAAAGAGAGTTGAAGATCATG15.555555555555555No Hit
CCCTTGTGAGTTGTAGTCATGGATGACTTCCCTCAGCTCATGCTGGATAC15.555555555555555No Hit
AGTGAAGTCAGAGAGGCAGACATGAACATAGCACATTCACAGCAGGCTGA15.555555555555555No Hit
CTGTCTATGTACTAGACATTGGAGATAAAAAATATTAATGAAACAGAGTA15.555555555555555No Hit
AACAAAAGCATTGTAGAAAAGTTTTTCAATCTACCAAACTTAAAAATGTC15.555555555555555No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT15.555555555555555No Hit
ATCTTACACAATCTCCCATGATAGAGAAATACACTCAGAGGTATACATAG15.555555555555555No Hit
GTTGTACTGCTCAGGACAGGCTATGGTTCTGTAAATGAACAAATGAAAAC15.555555555555555No Hit
GGAGGGAGGCGCAGGGGGGTAGGCGAAGGCACGCATCGCCGGGCGCCCGG15.555555555555555No Hit
CAAAATCGGACATTTAATAATCAAGATAAAATAACTTCAACCAATAGGAA15.555555555555555No Hit
ATATAATCGACTGAACATTAATGTCATAATAAACGAATGTAATTGCCGTT15.555555555555555No Hit
CGATTGGTTTGTGAATACTGATTAAATTATGAGTGTGCAATTTGCATTGC15.555555555555555No Hit
TTAAAAGATATTTCTTATTAATTGAGTTTCTATTGTAAAAGTTCCCTACA15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)