FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16684324
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA320350.014.7075211
GTATAGA111900.014.59728051
TATACTG165000.014.4946715
GTATTAG126250.013.915651
GTATAGG66500.013.8644171
GTATTAT189950.013.1473641
GTACTAG57050.012.852431
GTATTAA170950.012.6126661
TCTATAC114450.012.6035743
GTCCTAG83450.012.4402531
GTATAAG106050.012.3220051
TACACTG157050.011.9058425
CCTATAC69450.011.7940413
TTATACT185900.011.6180554
GTGTATA209250.011.5877881
CTATACT120900.011.5698464
GTGTAAG119150.011.5156191
GTCCTAC56250.011.4864821
GTATAAT182900.011.4710631
GTCCTAA90550.011.4648161
Produced by FastQC (version 0.11.5)