FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15320978
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG108900.016.199291
GTATAGA96550.016.0156611
TATACTG117600.014.8525025
GTATTAA138450.014.5246071
GTATATA195800.014.3858861
GTATTAT144350.014.23269751
TTATACT145550.012.8970864
GTGTATA152450.012.8098021
GTATAAT136450.012.7157491
TAATACT138500.012.6116324
GTATAAG100400.012.3645971
TCTATAC87350.012.19982053
GTATAGG62450.012.0897831
ATAATAC148650.011.8018333
TATATAC199150.011.6484643
ATTATAC134300.011.6054363
GTATAAA178400.011.5568921
TATACTA105500.011.5410865
ATATACT158600.011.5159934
TATAATA227350.011.4536492
Produced by FastQC (version 0.11.5)