FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCCTGACTTCTGCTATCTGTAAACTTTTCTTCTGGTACCTGCAGTGCT13.4482758620689653No Hit
GCCCGTGACCGGTGACTGGCCTAAGGAAACACCTGAGTAAGTGGGGGCTG13.4482758620689653No Hit
ACTTAAAACCACTTACAATAAACTTGGGAAACTACCGTCAGCTGAGTTCA13.4482758620689653No Hit
CTTCTAAGTCGCAATATTATTGGGACGCTCGATTTAATGAAACTGAACCT13.4482758620689653No Hit
GGATATTTCTCCACTCCAAATCTCATGTTATAATGTAATCCCCAGTGTTG13.4482758620689653No Hit
GGCAAAGACTTCATTACAAAAAAAAAAAAAAAAGCAATTGCAACAAAAAC13.4482758620689653No Hit
CTTCAAGCATATCCGCATTGTTATTTGATGCAGAACCATCTGTACCAAGA13.4482758620689653No Hit
AGCTATACCTAGAGCCCTTCTTTGCCAAAGAAGGATGTATTCAACCTTTA13.4482758620689653No Hit
TGCCAACGCAGTGCTCGCTCAGTCCCTCGAAGTGTGTTTAGAGGACACCA13.4482758620689653No Hit
GTTGAAATGATGACCAAAGCTGAATTCTGTCCAAGTTTGCAAATCTCATG13.4482758620689653No Hit
AATATGTATTCAATCAACGAGGTGAGTGAAAGTGACTAGCTGAACGCCCA13.4482758620689653No Hit
GTACGATAGTATACAATCAGTGATAAGCCTGATCACAGAGTTGCCTCCGG13.4482758620689653No Hit
GTACATAGAACTGCGCAAAGCGGTATGCTGTCAGACACTAAAAAAAAAAT13.4482758620689653No Hit
CCCAATTAACTTCCTACATACCATTTTCCAATCTTTCCAGGGAAACTGAG13.4482758620689653No Hit
TGTCTGCATGGTTCTGTATCCTAAATCCATTGCGAAGGGTATCTAGAAAG13.4482758620689653No Hit
CCCATTTACTTATCAAAATTCTGGAAAAAATAATCAGTTTGTATCCCAAC13.4482758620689653No Hit
CCTCCCACCTCAGCCTCTTGAGTAGCTGGGACTACAGGTACATGCCACCA13.4482758620689653No Hit
AAACAAGACTGTTGAAGATAAATTCCCCTACACTCCCTTGTACCTACTCC13.4482758620689653No Hit
GGGGTGCACGAGGGGCGGGGGCGCTGGGAGCTGGATGGGGTGGGACCGTC13.4482758620689653No Hit
GAATTTCGCAGACGAGGTCTCTCTGGGGACGTCTAGCCCACGAGACCTGA13.4482758620689653No Hit
CAAGAATCGCGAGAACCCACAAAGAAGAGCTTCAACAAAGAAGACAACAT13.4482758620689653No Hit
AAACTTGTGACTCCCTAAGAACAAATGTCCTACAAGTGTAACAAAAAAAG13.4482758620689653No Hit
AACTGTTAAAAAAATAAAAAACAAAAAAATAAAAATAGAAAGAAAAAAAT13.4482758620689653No Hit
GGGTTATGGCGGGCCCGCTCTTCGGGCTGCTTGGCGGGCCGGGAGCGCTG13.4482758620689653No Hit
AACTTATTTAGTGTTTGTACCATGGTGAGAAAGAACTAAAATTCTCACAC13.4482758620689653No Hit
GGGAATCACTCCGACCTCATCGCTCTCACCATCTGGTGTAACCTGCCTGG13.4482758620689653No Hit
CCATTACAGAATTATCAACTGAGTGGATATAATTCTATTATGGAGGAGTT13.4482758620689653No Hit
GCTCTGGGAAGTGGTGTCCTCTGTTTGGCACCATACAAAAATCCAGAGAA13.4482758620689653No Hit
CGAGAGTCATGCCAATTGCGTAATGGGCCAAATCCAATAGCGCAGATTTC13.4482758620689653No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)