FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15164813
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA227550.015.8571181
GTATTAG112750.015.8402941
GTATAGG72050.015.4170811
GTATAGA107350.014.1347761
GTATAAT158500.014.1080241
TATACTG131550.013.8288225
TATACAG134550.013.5204875
GTATTAA150250.013.191461
GTATTAT181150.013.10551551
GTATAAG105400.012.6053571
TCTATAC105300.012.4609143
CTATACT104850.012.4429154
CTAATAC105600.012.0136183
GTATAAC74450.011.9945821
TATATAC227650.011.9416223
GTGTATA143050.011.52080251
TTATACT164900.011.5159224
GTATAAA202500.011.4728061
GTCCTAG60400.011.4190861
TAATACT150950.011.37976554
Produced by FastQC (version 0.11.5)