FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4967663
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG4802.4062047E-615.0996754
GTATTAA47400.014.2443991
GTATATA91750.014.2431481
GTGTATA63050.013.8176981
TATACTG47350.013.7757075
GTCCTAC16000.013.6125921
GTATAGG19150.013.2690171
TAAGCGG3300.00886542813.1773685
GGACCGC4503.546209E-412.8844086
GTATAAG33950.012.6168731
ATATACT66150.012.4906164
GTCCTAT23350.012.43691351
TATACAG46900.012.3625635
GTATTAG35900.012.3360151
CTAGACT28900.012.2887334
TCTATAC36450.012.1320163
GTATAGA32400.012.100081
CCTATAC21000.012.0822943
GTGTTAG37550.011.9872961
CGTACCA7301.1359971E-611.9137855
Produced by FastQC (version 0.11.5)