FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948454
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG2455.12454E-520.7085635
GTACTAG2608.025321E-519.5354651
GTATAGG4152.466004E-819.232851
CTATACC5158.076313E-1018.2959144
ACTATAC4806.686605E-918.1199913
TACACGC3002.42181E-416.9119915
TAGACTG4307.623803E-716.8558065
TCTATAC5703.1122909E-916.530523
ATTAGAC3956.350643E-616.5144213
GTGTATA7601.8189894E-1216.2305931
GTATACA5955.4169504E-915.853511
TATACAG6651.4515535E-915.2589415
GTATTAG5908.3811756E-814.7580261
TCTGGAC5908.49559E-814.7416893
GTATATA12050.014.4518431
GGTATAC3050.00525661714.2583558
ATACCGG3100.00585510614.028386
TAACACG4151.783222E-413.9720424
GTGCTAA5751.0079093E-613.8811011
GGACCGC5751.0205767E-613.8657336
Produced by FastQC (version 0.11.5)