FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18616050
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG174200.015.5604115
GTATATA341750.014.7443271
GTGTATA232350.014.4681271
GTATTAG136450.014.2605161
GTATAGG80100.013.9592541
TATACCG15950.013.6319565
GTATAAG128300.013.2988831
GTCCTAG95600.013.2908931
GTATTAT211550.013.2822581
TCTATAC125300.012.9598033
CTATACT128300.012.9367474
TACACTG177350.012.5868515
CCTATAC70100.012.5132113
GTATATG203950.012.4956081
TATACAG172550.012.3909515
GTATTAA184800.012.3760651
GTGTAGG94650.012.3503491
GTATAGA126450.012.1728161
GTCTTAG115400.012.0800691
GTCCTAT85700.012.030441
Produced by FastQC (version 0.11.5)