FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_132_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_132_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11032885
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA166100.016.565741
TATACTG103450.013.6607695
GTATAAG87400.013.4568961
GTATTAG88000.013.4476451
TATACAG106600.013.3250825
GTATAGA90300.013.0247251
GTATAGG61400.012.88841151
GTATATG117400.012.3681071
CCTATAC57700.012.312253
GTATTAT149300.012.1082351
GTATAGT77150.011.9511341
GTATAAT124850.011.9208331
TTATACT133900.011.7994224
GTACTAG33000.011.6601251
GTATACA85850.011.5856811
GTCTTAG63450.011.5566251
GTATTAA126250.011.4436081
ACTGATC73250.011.179858
CTATACT88100.011.1056184
TATATAC164650.010.8748283
Produced by FastQC (version 0.11.5)