FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14945072
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA5509.822543E-1118.449976145
CCTATAC61300.014.6647433
TATACTG164250.014.0760535
GTATATA322700.013.74821951
GTATTAG110000.013.6641251
GCCGACC11350.013.410775145
CCTACAC71350.012.903963
GTCCTAT67750.012.5395151
CTATACT112850.012.5240034
GTATTAT186250.012.4754971
GTGTATA203950.012.2116571
TCTATAC108100.012.1385323
GTCCTAA82250.012.0945251
GTATTAA155600.011.9929971
GTACTAG50600.011.9105481
TATACAG151150.011.7956685
GTATAGA108350.011.7947281
GTCCTAG70500.011.535411
CTATACA133250.011.3681344
TATACCG10858.8584784E-1011.3557215
Produced by FastQC (version 0.11.5)