FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_123.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_123.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59.803921568627452No Hit
GCCATTGCTTTTGGTGTTTTAGACATGAAGTCCTTGCTCATGCCTATGTC11.9607843137254901No Hit
GTGCCAAGTGATCACGGATAGTGGTCGGCGAGGCATGTGGGGTTCAAATG11.9607843137254901No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG11.9607843137254901No Hit
GATTGCTAAAGATTAGAATCACCTGGGGAGCTTTTAAAACTTCTAATGCC11.9607843137254901No Hit
ATTCCAGTCCATTCCACTCGAGTCCATTCCATTCGAGTCTATTCCATTCC11.9607843137254901No Hit
ATGAAAACGTACTGTGATATTATTCATAATATCCTTGGAAAATGGGACTG11.9607843137254901No Hit
GGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGCCGGGGGGGGGCGGT11.9607843137254901No Hit
ATACTAGAAAGAAATTCCCCGTCTTGTGAAACATTTCTTGTGAAATGTTG11.9607843137254901No Hit
GATGAAGACAGTAGGCTTGAACATGACCAAGTCAAATTCCCCTAATGTCC11.9607843137254901No Hit
GGTGTGGTGGTGCATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAG11.9607843137254901No Hit
CACAAAATATTGTTATAATCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.9607843137254901No Hit
GGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.9607843137254901No Hit
CTTCGTGCCGTAGACCTGTGGAGAAGAGATTAAAATGCCACCAAAAGAAA11.9607843137254901No Hit
AAGGAAGTCCTTGAAAATACTCAGAGAAATTGCCAAAGTAATAATCATGG11.9607843137254901No Hit
AATTAAACGAGTGTGGTACTGTCATACAGACATTCAGATAAATGGAATAG11.9607843137254901No Hit
GTCTTACACTGTATAGAAAAGGCAAATCCCTGTTAAAGGAGGCCACTTTA11.9607843137254901No Hit
GGCGTACAGAGTAAGCTCGGTTACTCTGTCTTACTTGGAAGCGCAAATAA11.9607843137254901No Hit
CTTATGTTCGGTGGAGTTATTGCTCCTTTATGAGGTTTACTTTTGGGTCT11.9607843137254901No Hit
ATTTATGACAAATAAAATTAAGCAACAAGAATCATGAAAGCAAAAAAAAA11.9607843137254901No Hit
TTATTAGCTAGGCATGGTGGCATGCACCTGTACTTCCAGCTGCTCAGGAG11.9607843137254901No Hit
GAATAGCCCCATTTTCTTGTACCTGTCCTGATGGGCCCAATTTACATTCC11.9607843137254901No Hit
GCTATAAAGACACATGCACACATATGTTTATTGTGGCACTATTCACAATA11.9607843137254901No Hit
ACTGATATAAGGATATCACCACTGATCCCACAGAATCAGTGATCTCCCTT11.9607843137254901No Hit
TATTTGATGAGGCCGAGAACCCCAAAGGAATTGTTTTTGCCATCGAAATG11.9607843137254901No Hit
GCAATTATGTTTAAACAGAAGCGGGGGCTAGGGTGGAGGCCCAAGCCCTT11.9607843137254901No Hit
ATGTAAACTGTAGTAAGTTAGCAGAACCAATCCGTAAAGTTTTTACATTG11.9607843137254901No Hit
GTGCTAGGGGTGCGGAAAGAGGCGCTATTAGAGAAGGGCTACCCGACTGT11.9607843137254901No Hit
ATTGGGGTCCGTTTGGTTGTTGGGGTCGGTTCGGGGGGGTGGGGGGGGGG11.9607843137254901No Hit
CTCCTAGTCCAGAGGCGGCGGCAGGGGCATTGCGTAAGCCCCGGGGGTCC11.9607843137254901No Hit
GAATAGAAGTGGTCAGAATTCTTGCTTGGTTCTTTCTCAGAGGAAGCTTT11.9607843137254901No Hit
GATTTCATCAAAAAATATTTGCACAAGCCATTGCTTCAGTAACCACGTCT11.9607843137254901No Hit
GTCCTCCTCCTCCTCCTGTGGCACTCCCCCTTGGTTCTTCAGCAAACCAC11.9607843137254901No Hit
ATCCTGGTGTGAAATACTGAGTAGCTGCTGCCCACCAGGGAATTTGTGAA11.9607843137254901No Hit
CTGCAGGGGGCGGCAGAGGGTAGGCTGGGAGCCCTCTGCGGTCTCAGTCC11.9607843137254901No Hit
GTCTCTGATAAAACAGACTTTAAACCAACAAAGATCAAAAGCGACACAGC11.9607843137254901No Hit
CATTTTATTTATTTATTTTTTATTCCACCATATGTGATTCATGCTCATTG11.9607843137254901No Hit
GTGTATATAACTCTACTATTATTACTGGAAGAGACTCTGATGAAATCTGC11.9607843137254901No Hit
GTATTACAAGGACAACAACTGCCCCCCCTGGGCACCCTTGGACCCAAAGG11.9607843137254901No Hit
GCAATAATACCAAAGACCAGCTATATGAACAAAATAACATGCTAGATTAT11.9607843137254901No Hit
ACACAGATCATCCTGCATGTTCATCAACTCACAGAGTATTTCACATATTA11.9607843137254901No Hit
CTGCATGCCTGTGCTCTTGGCTCTATGTGCTTTTTGTCTAAGAGCTCACA11.9607843137254901No Hit
CTCAGTTTCCCCATATATAAAATGTAGATGGTAATAGTAACTACCTCAAC11.9607843137254901No Hit
GCACACTATAGCTCATGGGCCAAGTGAATAGCAGCGTAATATTTCAATAT11.9607843137254901No Hit
ACAGAGCTTAGACCAATTTTAATGGGACTTACACTTTCTGTTATTATGGG11.9607843137254901No Hit
CACAAGAACAGCCTAACACACCCACCTTCACTGGCAATTAAAGGGATTAG11.9607843137254901No Hit
GTACAGAAGTTCCCTACTTAAAAAAGATTTTCCATATGCTCTACACCAGT11.9607843137254901No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTGC50.0145.02
TGCCGTA50.0145.06
CGTAGAC50.0145.09
TCGTGCC50.0145.03
CCGTAGA50.0145.08
CACCTCA50.0145.0145
CGTGCCG50.0145.04
CTTCGTG50.0145.01
GCCGTAG50.0145.07
GTGCCGT50.0145.05
Produced by FastQC (version 0.11.5)