FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18184978
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA118100.015.24737551
GTATATA325100.014.1377421
CCTATAC72600.014.0792313
TATACTG166800.013.904335
GTATTAT200200.013.8545561
CTATACT130600.013.4302424
GTATAGG74800.013.2987951
GTATTAG126900.013.160111
GTATTAA177300.013.1049251
GTGTATA215800.012.6174961
GTATAAG117900.012.2555431
TCTATAC116500.012.0718053
TATACAG171350.012.0547125
GTATAAT189350.011.9258231
ACTGATC150150.011.7292728
TTATACT200150.011.6603584
GTCTAAG96450.011.5934261
GTACTAG60050.011.4869291
TAGACTG132250.011.3441185
TACACTG172400.011.308665
Produced by FastQC (version 0.11.5)