FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n02_S_114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n02_S_114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15849136
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG114150.014.5014551
GTATATA263900.014.0308331
GTGTATA181100.013.5904521
TATACTG137700.013.1053965
TCTATAC104300.012.92762853
GTATAGG66200.012.7219191
GTATTAT165700.012.2684081
TATACAG147350.012.0995645
GTATTAC83600.012.0714981
GTATAGA103150.012.0359271
TACACTG153950.012.0045315
TAATACT156650.011.9367574
GTATAAG99150.011.935691
GTCTTAG98100.011.7674061
CTATACT111050.011.6823854
GTATTAA155050.011.6595081
CTAATAC114850.011.6138733
ATTATAC157600.011.5913653
ACTGATC119200.011.4304928
GTCCTAT72350.011.3394651
Produced by FastQC (version 0.11.5)