FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57005557
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5037200.883633151764485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC21750.018.730284145
GTATATA123850.016.5132181
GACGCGC21700.014.41532145
GTATTAG51500.014.082231
GTATAGA55750.013.5290441
GCCACGC30550.013.096856145
GTGTATA82900.012.8600131
TATACTG67950.012.774115
CCTATAC34800.012.6793543
TATATAC127300.012.4440633
TATACAG69600.011.951645
CTAATAC61500.011.7617353
GTATAAA109800.011.5588431
GTATTAT93050.011.535171
CTAGTAC25300.011.4363113
GTACTAT38950.011.3579771
GTATAGG35600.011.2044711
GTGTAAG54100.011.1265231
TAATACT83450.011.0949454
GTATTAC44450.011.0947061
Produced by FastQC (version 0.11.5)