FastQCFastQC Report
Mon 28 Jan 2019
HGY2NBGX9_n01_S_76_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGY2NBGX9_n01_S_76_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15307565
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG63950.015.4186341
GTATTAG103350.015.2930311
GTATAGA102350.014.4507331
TATACTG129650.013.8125815
GTATATA244900.013.6773121
GTATAAG95750.013.6295421
CTAATAC101750.012.6834093
GTGTATA173800.012.5980911
GTATTAT157200.012.4525641
TATACCG17500.012.42892555
CCTATAC61150.012.2121233
GTCCTAG77700.012.0369441
TCTATAC96850.011.9776273
GTATTAA146800.011.655571
CTTACAC101350.011.5173493
GCTATAC56850.011.47790053
GTCCTAA77150.011.3709561
GTCTAAG77250.011.3562371
GTGTAAG104600.011.298051
GTCCTAC54100.011.2571791
Produced by FastQC (version 0.11.5)